Acoustic liquid dispensing apparatus

• US 8,171,798 B2
• Filed: 08/25/2008
• Issued: 05/08/2012
• Est. Priority Date: 12/22/2004
• Status: Active Grant

• Current Assignee: Labcyte, Inc. (Danaher Corporation)
• Original Assignee: EDC Biosystems, Inc.
• Inventors: Van Tuyl, Michael R., Chiao, James
• Primary Examiner(s): Williams, Hezron E
• Assistant Examiner(s): Shah, Samir M
• Application Number:
  US12/197,985
• Publication Number:
  US 20090060796A1
• Time in Patent Office: 1,352 Days
• Field of Search: 73/645.3, 73/32.A, 735/99, 736/02, 735/97, 735/89, 222/420
• US Class Current: 73/597
• CPC Class Codes: G01N 11/10 by moving a body within the material
• CPC Class Codes: G01N 2291/02809 Concentration of a compound, e.g. measured by a surface mass change
• CPC Class Codes: G01N 2291/02818 Density, viscosity
• CPC Class Codes: G01N 2291/02836 Flow rate, liquid level
• CPC Class Codes: G01N 2291/02881 Temperature
• CPC Class Codes: G01N 2291/044 Internal reflections (echoes), e.g. on walls or defects
• CPC Class Codes: G01N 29/024 by measuring propagation velocity or propagation time of acoustic waves
• CPC Class Codes: G01N 29/028 by measuring mechanical or acoustic impedance
• CPC Class Codes: G01N 29/032 by measuring attenuation of acoustic waves
• CPC Class Codes: G01N 29/221 Arrangements for directing or focusing the acoustical waves electronic orientation or focusing G01N29/262; sound directing or focusing G10K11/26; mechanical steering of sound transducers or their beams G10K11/35
• CPC Class Codes: G01N 29/222 Constructional or flow details for analysing fluids optoacoustic fluid cells G01N29/2425
• CPC Class Codes: G01N 29/343 pulse waves, e.g. particular sequence of pulses, bursts
• CPC Class Codes: G01N 9/24 by observing the transmission of wave or particle radiation through the material

Abstract

A liquid dispensing apparatus for dispensing droplets of a liquid, and methods for measuring various fluid parameters of the liquid are described. The liquid dispensing apparatus comprises a container having a chamber for holding a liquid. An orifice is positioned at an end of the chamber for dispensing droplets of the liquid, the orifice being configured to retain the liquid in the container if the container is positioned with the orifice facing in a downward direction. An acoustic transducer means is at least partially positioned in the chamber for periodically propagating a focused acoustic beam toward the orifice and through at least some of the liquid while the liquid is contained in the chamber, with the focused acoustic beam being capable of causing a droplet of the liquid to be ejected from the orifice when a free surface of the liquid is within the depth of field of the acoustic transducer means. Fluid parameters that can be measured include the sound velocity in the liquid, liquid level and liquid concentration, liquid acoustic impedance, liquid density ultrasonic attenuation and ultrasonic viscosity.

Claims

1. A liquid dispensing apparatus comprising:

a container having a chamber adapted for holding a liquid;

an orifice positioned at an end of the chamber for allowing a free surface of the liquid to form when the liquid is held in the chamber, the free surface being the region where the liquid interfaces with the environment outside of the container, the orifice being configured to retain the liquid in the container if the container is positioned with the orifice facing in a downward direction and the liquid is being held in the chamber;

an acoustic transducer means at least partially positioned in the chamber for periodically propagating a focused acoustic beam toward the orifice and through at least some of the liquid when the liquid is being held in the chamber, the focused acoustic beam being capable of causing a droplet of the liquid to be ejected from the free surface of the liquid when the focused acoustic beam is focused at or near the free surface; and

a calibration surface positioned in the chamber for intercepting a part of the focused acoustic beam to generate a reflected acoustic wave.

2. The apparatus of claim 1 wherein the volume of the droplet is in the range of one picoliter to one microliter.

3. The apparatus of claim 1 wherein the volume of the droplet is in the range of five picoliters to twenty-five picoliters.

4. The apparatus of claim 1 wherein the volume of the droplet is approximately one nanoliter.

5. The apparatus of claim 1 wherein the orifice is circular in shape and has a diameter in the range of 0.2 to 1.5 millimeters.

6. The apparatus of claim 1 wherein the acoustic transducer means comprises a piezoelectric crystal attached to a first end of an acoustic waveguide, the acoustic waveguide having a lens formed in a second end of the acoustic waveguide for focusing the acoustic beam on the free surface.

7. The apparatus of claim 6 wherein the lens has a focal distance in the range of one to twelve millimeters.

8. The apparatus of claim 1 further comprising:

a waveguide housing for surrounding the acoustic transducer means and protecting the acoustic transducer means from the liquid, the waveguide housing having one or more channels positioned about the outside of the waveguide housing for allowing the liquid to flow past the waveguide housing.

9. The apparatus of claim 1 further comprising:

a temperature sensing means for determining the temperature of the liquid in the chamber.

10. The apparatus of claim 1 further comprising:

a nozzle extending from the container, the nozzle including part of the chamber but having a first width that is less than a second width of the chamber, the orifice being positioned at a distal end of the nozzle.

11. The apparatus of claim 10 wherein the calibration surface is positioned in the nozzle.

12. The apparatus of claim 10 wherein the nozzle includes a tip at a distal end of the nozzle, the tip having a third width that is less than the first width and the orifice is positioned in the tip.

13. The apparatus of claim 10 further comprising:

a suction means for drawing a volume of the liquid held in an external location up into the chamber through the nozzle.

14. The apparatus of claim 10 further comprising:

an external liquid receptacle connected to the chamber for delivering the liquid to the chamber.

15. The apparatus of claim 14 wherein the free surface of the liquid is positioned in the nozzle and the external liquid receptacle can be raised or lowered relative to the chamber so as to change the position of the free surface of the liquid in the nozzle.

16. The apparatus of claim 14 further comprising:

a volume displacement means associated with the external liquid receptacle for controlling the flow of the liquid into the chamber or for controlling the liquid pressure within the chamber.

17. A liquid dispensing apparatus comprising:

a container having a chamber adapted for holding a liquid;

an orifice in the chamber for allowing a free surface of the liquid to form when the liquid is held in the chamber, the free surface being the region where the liquid interfaces with the environment outside of the container, the orifice being sized to retain the liquid in the container if the container is positioned with the orifice facing in a downward direction and the liquid is being held in the chamber; and

an acoustic transducer means at least partially positioned in the chamber for periodically propagating a focused acoustic beam toward the orifice and through at least some of the liquid when the liquid is held in the chamber, the acoustic transducer means comprising a lens configured to focus the focused acoustic beam on the free surface when the liquid is held in the chamber;

wherein droplets having a volume of less than one nanoliter are ejected from the free surface when the focused acoustic beam is propagated through the liquid.

18. The liquid dispensing apparatus of claim 17 wherein the lens has an f-number in the range of one to four.