Biomaterial measuring device and manufacturing method thereof

• US 8,172,994 B2
• Filed: 12/10/2004
• Issued: 05/08/2012
• Est. Priority Date: 12/11/2003
• Status: Active Grant

• Current Assignee: Ceragem Medisys Inc.
• Original Assignee: Ceragem Medisys Inc.
• Inventors: Lee, Jin-Woo
• Primary Examiner(s): Barton, Jeffrey T
• Assistant Examiner(s): RUFO, LOUIS J
• Application Number:
  US10/582,529
• Publication Number:
  US 20100062520A1
• Time in Patent Office: 2,706 Days
• Field of Search: 204/403.02, 204/403.03, 204/403.04, 204/403.14, 204/403.01, 204/403.15, 298/76
• US Class Current: 204/403.03
• CPC Class Codes: G01N 2021/7783 Transmission, loss
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/78 producing a change of colour
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: Y10T 29/49208 by assembling plural parts

Abstract

A method of producing a biomaterial measuring device is disclosed. The method includes forming a plurality of reactions elements, to which an assay reagent is applied, on a first substrate, cutting the resulting first substrate in a unit of individual reaction element, and attaching a first substrate piece, which is formed by cutting the resulting first substrate in the unit of individual reaction element, to a predetermined portion of a second substrate. In the biomaterial measuring device, since a material cost in minimized and it is easy to automate production, it is possible to reduce a production, it is possible to reduce a production cost.

Claims

1. A method of producing a biomaterial measuring device that uses an electrochemical assay, comprising:

forming a plurality of reaction elements on a first substrate, wherein forming a reaction element comprises;

forming at least two first electrodes on a first side of the first substrate,

forming a second electrode on a second side of the first substrate, wherein the second electrode transmits an electrical signal to a measuring device,

forming a via hole through the first substrate,

electrically connecting, through the via hole, the first electrodes on the first side of the first substrate to the second electrode on the second side of the first substrate, and

applying an assay reagent to the first electrodes on the first side of the first substrate;

cutting the first substrate into a plurality of reaction elements;

forming at least one cavity, each with space for a capillary, on one side of a second substrate; and

forming at least one capillary, by attaching the first side of at least one reaction element into at least one of the cavities in the second substrate,

wherein each capillary is for feeding a biomaterial therethrough.

2. The method as set forth in claim 1, wherein the plurality of reaction elements are attached to the second substrate.

3. The method as set forth in claim 1, further comprising mounting a dehumidifying agent for removing moisture, which is introduced from an environment to the assay reagent, on a predetermined portion of the biomaterial measuring device while the dehumidifying agent is isolated from the environment.

4. A biomaterial measuring device that uses an electrochemical assay, comprising:

a first reaction element comprising;

a first substrate,

a plurality of first electrodes on a first side of the first substrate,

a second electrode on a second side of the first substrate,

a via hole through the first substrate for electrically connecting the electrodes on the first side of the first substrate to the second electrode on the second side of the first substrate,

an assay reagent applied to the first electrodes; and

a second substrate having at least one cavity, each with space for a capillary, on one side of the second substrate, wherein the first side of the reaction element is mounted into one of the at least one cavities in the second substrate, and

a capillary, formed by mounting of the reaction element one of the at least one cavities in the second substrate,

wherein the assay reagent reacts with a biomaterial introduced through the capillary to generate an electrical signal, and

wherein the generated electrical signal is transmitted to a measuring device through the second electrode.

5. The biomaterial measuring device as set forth in claim 4, wherein the second substrate is made of any one selected from the group consisting of plastic, glass, and semiconductor wafer.

6. The biomaterial measuring device as set forth in claim 4, further comprising a second reaction element, wherein the second reaction element comprises:

a first substrate,

a plurality of first electrodes on a first side of the first substrate,

a second electrode on a second side of the first substrate,

a via hole through the first substrate for electrically connecting the electrodes on the first side of the first substrate to the second electrode on the second side of the first substrate, and

an assay reagent applied to the first electrodes,

wherein the second reaction element substrate is mounted into one of the at least one cavities in the second substrate.

7. The biomaterial measuring device as set forth in claim 4, wherein the first substrate is formed of an insulating material.

8. The biomaterial measuring device as set forth in claim 4, wherein the second electrode on the second side of the first substrate is formed of an electrical conductive material.

9. The biomaterial measuring device as set forth in claim 4, further comprising a dehumidifying agent which is mounted on a predetermined portion of the biomaterial measuring device so that the dehumidifying agent is isolated from an environment and which removes moisture introduced from the environment to the assay reagent.

10. The biomaterial measuring device as set forth in claim 7, wherein the insulating material is one of a printed circuit board, a plastic substrate, a glass substrate, and a semiconductor wafer.