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Method and apparatus for analysis and sorting of polynucleotides based on size

  • US 8,173,001 B2
  • Filed: 01/11/2010
  • Issued: 05/08/2012
  • Est. Priority Date: 09/25/1996
  • Status: Expired due to Fees
First Claim
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1. A method for transporting molecules in a microfluidic device, said method comprising:

  • a) providing a sample comprising molecules;

    b) providing a microfluidic device comprising an inlet, a first microfluidic channel in fluidic communication with a second microfluidic channel, wherein said first and second microfluidic channels intersect at a junction, and wherein said first microfluidic channel is in fluidic communication with said inlet;

    c) transporting said sample into said inlet port and through said first microfluidic channel to said junction with a hydrostatic pressure differential;

    d) at said junction electrophoretically diverting said molecules from said first microfluidic channel into said second microfluidic channel;

    e) transporting said molecules through said second microfluidic channel; and

    f) detecting said molecules.

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