Method and apparatus for analysis and sorting of polynucleotides based on size
First Claim
Patent Images
1. A method for transporting molecules in a microfluidic device, said method comprising:
- a) providing a sample comprising molecules;
b) providing a microfluidic device comprising an inlet, a first microfluidic channel in fluidic communication with a second microfluidic channel, wherein said first and second microfluidic channels intersect at a junction, and wherein said first microfluidic channel is in fluidic communication with said inlet;
c) transporting said sample into said inlet port and through said first microfluidic channel to said junction with a hydrostatic pressure differential;
d) at said junction electrophoretically diverting said molecules from said first microfluidic channel into said second microfluidic channel;
e) transporting said molecules through said second microfluidic channel; and
f) detecting said molecules.
0 Assignments
0 Petitions
Accused Products
Abstract
The invention relates to a microfabricated device and methods of using the device for analyzing and sorting polynucleotide molecules by size.
-
Citations
5 Claims
-
1. A method for transporting molecules in a microfluidic device, said method comprising:
-
a) providing a sample comprising molecules; b) providing a microfluidic device comprising an inlet, a first microfluidic channel in fluidic communication with a second microfluidic channel, wherein said first and second microfluidic channels intersect at a junction, and wherein said first microfluidic channel is in fluidic communication with said inlet; c) transporting said sample into said inlet port and through said first microfluidic channel to said junction with a hydrostatic pressure differential; d) at said junction electrophoretically diverting said molecules from said first microfluidic channel into said second microfluidic channel; e) transporting said molecules through said second microfluidic channel; and f) detecting said molecules. - View Dependent Claims (2, 3, 4, 5)
-
Specification