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Method of determining metabolism of a test agent

  • US 8,173,361 B2
  • Filed: 02/17/2010
  • Issued: 05/08/2012
  • Est. Priority Date: 01/16/2003
  • Status: Active Grant
First Claim
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1. A method for detecting one or more metabolites of a test agent in a tissue comprising:

  • A) incubating a test agent and an enzyme within a three-dimensional structure comprising a first mold or polymer scaffold, a semi-permeable membrane, and a second mold or polymer scaffold, wherein the semi-permeable membrane is disposed between the first and second molds or polymer scaffolds, and wherein the first mold or polymer scaffold has microchannels that form a fluidic branching network that mimics the forces and transport of natural vasculatures and wherein the second mold or polymer scaffold comprises cells;

    B) forming an enzyme-substrate complex between the enzyme and the test agent; and

    C) detecting one or more metabolites of the test agent.

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