Nucleic acid-based tests for RHD typing, gender determination and nucleic acid quantification
First Claim
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1. A multiplex method for determining the presence or absence of one or more RhD exons in a fetal nucleic acid, comprising:
- simultaneously amplifying fetal nucleic acid from a pregnant female with two or more amplification primers yielding amplification products,contacting the amplification products with extension primers under extension conditions, thereby generating extension products, anddetermining the presence or absence of an RhD exon in the fetal nucleic acid based on the extension products;
wherein;
the extension primers independently comprise a nucleotide sequence of;
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Abstract
The invention in part provides nucleic acid-based assays, which are particularly useful for non-invasive prenatal testing. The invention in part provides compositions and methods for RhD typing, detecting the presence of fetal nucleic in a sample, determining the relative amount of fetal nucleic acid in a sample and determining the sex of a fetus, wherein each of the assays may be performed alone or in combination.
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Citations
9 Claims
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1. A multiplex method for determining the presence or absence of one or more RhD exons in a fetal nucleic acid, comprising:
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simultaneously amplifying fetal nucleic acid from a pregnant female with two or more amplification primers yielding amplification products, contacting the amplification products with extension primers under extension conditions, thereby generating extension products, and determining the presence or absence of an RhD exon in the fetal nucleic acid based on the extension products;
wherein;the extension primers independently comprise a nucleotide sequence of; - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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Specification