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Methods and reagents for preserving RNA in cell and tissue samples

  • US 8,178,296 B2
  • Filed: 08/03/2009
  • Issued: 05/15/2012
  • Est. Priority Date: 07/31/1998
  • Status: Expired due to Fees
First Claim
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1. A method of preserving RNA in an intact cell of a biological sample comprising:

  • preserving the RNA of an intact cell by contacting the intact cell of the sample with an RNA preservation composition comprising a chelator of divalent cations;

    a citrate buffer or an acetate buffer; and

    an ammonium sulfate salt at a concentration between 20 g/100 ml and a saturating concentration of the salt, wherein the intact cell remains intact and the RNA in the intact cell is preserved thereby.

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