Small volume in vitro analyte sensor and methods of making
First Claim
1. A method for determining a concentration of an analyte in a sample, comprising the steps of:
- contacting a sample with a sensor, wherein the sensor comprises;
a first electrode, a second electrode, and a third electrode;
a sample chamber for holding the sample fluid, the sample chamber comprising the first electrode, the second electrode, and the third electrode, wherein the first electrode is positioned upstream of the second electrode relative to a sample entry port and the third electrode is positioned downstream of the second electrode relative to the sample entry port; and
an analyte-responsive enzyme and a redox mediator disposed in the sample chamber;
determining when the sample contacts the first electrode and the second electrode;
determining when the sample contacts the first electrode and the third electrode;
applying a potential between the first and second electrodes to electrolyze the analyte, thereby generating an analyte signal; and
determining the concentration of the analyte using the analyte signal.
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Accused Products
Abstract
A sensor utilizing a non-leachable or diffusible redox mediator is described. The sensor includes a sample chamber to hold a sample in electrolytic contact with a working electrode, and in at least some instances, the sensor also contains a non-leachable or a diffusible second electron transfer agent. The sensor and/or the methods used produce a sensor signal in response to the analyte that can be distinguished from a background signal caused by the mediator. The invention can be used to determine the concentration of a biomolecule, such as glucose or lactate, in a biological fluid, such as blood or serum, using techniques such as coulometry, amperometry; and potentiometry. An enzyme capable of catalyzing the electrooxidation or electroreduction of the biomolecule is typically provided as a second electron transfer agent.
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Citations
19 Claims
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1. A method for determining a concentration of an analyte in a sample, comprising the steps of:
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contacting a sample with a sensor, wherein the sensor comprises; a first electrode, a second electrode, and a third electrode; a sample chamber for holding the sample fluid, the sample chamber comprising the first electrode, the second electrode, and the third electrode, wherein the first electrode is positioned upstream of the second electrode relative to a sample entry port and the third electrode is positioned downstream of the second electrode relative to the sample entry port; and an analyte-responsive enzyme and a redox mediator disposed in the sample chamber; determining when the sample contacts the first electrode and the second electrode; determining when the sample contacts the first electrode and the third electrode; applying a potential between the first and second electrodes to electrolyze the analyte, thereby generating an analyte signal; and determining the concentration of the analyte using the analyte signal. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification