Amniotic membrane preparations and purified compositions and anti-inflammation methods
First Claim
1. A method for reducing inflammation in a subject in need thereof comprising, administering to the subject a pharmaceutical formulation comprising:
- (a) an AM preparation prepared from pulverized or around amniotic membrane isolated from frozen or previously frozen placenta comprising;
(i) high molecular weight hyaluronan (HA) that is cross-linked by a covalent bond to the heavy chain of inter-α
-trypsin inhibitor (Iα
I), the high molecular weight HA having a molecular weight greater than 1000 kDa;
(ii) tumor necrosis factor-stimulated gene 6 (TSG-6);
(iii) pentraxin (PTX-3); and
(iv) thrombospondin (TSP-1); and
(b) a pharmaceutically acceptable diluent, excipient, or carrier;
wherein the ratio of total protein to HA in the formulation is less than 100 parts total protein to 1 part HA; and
wherein inflammation is reduced.
5 Assignments
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Accused Products
Abstract
Compositions having a combination of specific biological components have been found to exert a number of useful effects in mammalian cells, including modulating TGF β signaling, apoptosis, and proliferation of mammalian cells, as well as decreasing inflammation in mice. These components can be obtained commercially, or can be prepared from biological tissues such as placental tissues. Placental amniotic membrane (AM) preparations described herein include AM pieces, AM extracts, AM jelly, AM stroma, and mixtures of these compositions with additional components. The compositions can be used to treat various diseases, such as wound healing, inflammation and angiogenesis-related diseases.
126 Citations
7 Claims
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1. A method for reducing inflammation in a subject in need thereof comprising, administering to the subject a pharmaceutical formulation comprising:
-
(a) an AM preparation prepared from pulverized or around amniotic membrane isolated from frozen or previously frozen placenta comprising; (i) high molecular weight hyaluronan (HA) that is cross-linked by a covalent bond to the heavy chain of inter-α
-trypsin inhibitor (Iα
I), the high molecular weight HA having a molecular weight greater than 1000 kDa;(ii) tumor necrosis factor-stimulated gene 6 (TSG-6); (iii) pentraxin (PTX-3); and (iv) thrombospondin (TSP-1); and (b) a pharmaceutically acceptable diluent, excipient, or carrier; wherein the ratio of total protein to HA in the formulation is less than 100 parts total protein to 1 part HA; and
wherein inflammation is reduced.- View Dependent Claims (2, 3, 4, 5, 6, 7)
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Specification