Methods for indexing samples and sequencing multiple polynucleotide templates
First Claim
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1. A method for sequencing nucleic acid sequences and identifying subsets of nucleic acid sequences, each subset of nucleic acid sequences isolated from a different source, the method comprising the steps of:
- a) ligating to each end of each nucleic acid sequence fragment in at least two samples, each sample isolated from a different source, a universal adaptor to generate adaptor-target-adaptors, wherein one of the adaptors comprises a sample specific tag sequence that differentiates adaptor-target-adaptors originating from different samples;
b) pooling the adaptor-target-adaptors of the different samples;
c) immobilizing the adaptor-target-adaptors on a surface;
d) sequencing the immobilized adaptor-target-adaptors on the surface to determine a sequence read of each immobilized adaptor-target-adaptor by hybridizing a first sequencing primer to the adaptor-target-adaptors, performing a first sequencing read and removing the first sequencing primer;
e) sequencing the sample specific tag sequence of each immobilized adaptor-target-adaptor by hybridizing a second sequencing primer to the adaptor-target-adaptors and performing a second sequencing read,wherein steps d) and e) determine a nucleic acid sequence of the immobilized adaptor-target-adaptors and identifies the immobilized adaptor-target-adaptors as a member of a subset of nucleic acid sequences.
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Abstract
The invention relates to methods for indexing samples during the sequencing of polynucleotide templates, resulting in the attachment of tags specific to the source of each nucleic acid sample such that after a sequencing run, both the source and sequence of each polynucleotide can be determined. Thus, the present invention pertains to analysis of complex genomes (e.g., human genomes), as well as multiplexing less complex genomes, such as those of bacteria, viruses, mitochondria, and the like.
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18 Claims
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1. A method for sequencing nucleic acid sequences and identifying subsets of nucleic acid sequences, each subset of nucleic acid sequences isolated from a different source, the method comprising the steps of:
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a) ligating to each end of each nucleic acid sequence fragment in at least two samples, each sample isolated from a different source, a universal adaptor to generate adaptor-target-adaptors, wherein one of the adaptors comprises a sample specific tag sequence that differentiates adaptor-target-adaptors originating from different samples; b) pooling the adaptor-target-adaptors of the different samples; c) immobilizing the adaptor-target-adaptors on a surface; d) sequencing the immobilized adaptor-target-adaptors on the surface to determine a sequence read of each immobilized adaptor-target-adaptor by hybridizing a first sequencing primer to the adaptor-target-adaptors, performing a first sequencing read and removing the first sequencing primer; e) sequencing the sample specific tag sequence of each immobilized adaptor-target-adaptor by hybridizing a second sequencing primer to the adaptor-target-adaptors and performing a second sequencing read, wherein steps d) and e) determine a nucleic acid sequence of the immobilized adaptor-target-adaptors and identifies the immobilized adaptor-target-adaptors as a member of a subset of nucleic acid sequences. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method for sequencing nucleic acid sequences and identifying subsets of nucleic acid sequences, each subset of nucleic acid sequences isolated from a different source, the method comprising the steps of:
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a) ligating to each end of each nucleic acid sequence fragment in at least two samples, each sample isolated from a different source, a universal adaptor to generate adaptor-target-adaptors; b) amplifying the adaptor-target-adaptors with at least two primers to generate amplified adaptor-target-adaptors, wherein one of the amplification primers comprises a sample specific tag sequence that differentiates amplified adaptor-target-adaptors originating from different samples; c) pooling the amplified adaptor-target-adaptors of the different samples; d) immobilizing the amplified adaptor-target-adaptors on a surface; e) sequencing the immobilized adaptor-target-adaptors on the surface to determine a sequence read of each immobilized adaptor-target-adaptor by hybridizing a first sequencing primer to the adaptor-target-adaptors, performing a first sequencing read and removing the first sequencing primer; f) sequencing the sample specific tag sequence of each immobilized adaptor-target-adaptor by hybridizing a second sequencing primer to the adaptor-target-adaptors and performing a second sequencing read, wherein steps e) and f) determine a nucleic acid sequence of the immobilized adaptor-target-adaptors and identifies the immobilized adaptor-target-adaptors as a member of a subset of nucleic acid sequences. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18)
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Specification