Temperature resistant pH buffers for use at low temperatures
First Claim
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1. A composition made by a method comprising:
- selecting a pH of the composition, the composition having a pH of 6 to 8;
selecting a first buffer with a negative temperature coefficient, the first buffer comprising HEPES or BisTrisPropane;
selecting a second buffer with a positive temperature coefficient, the second buffer comprising potassium phosphate; and
forming the composition comprising the first buffer and the second buffer;
wherein the composition does not comprise a pH-indicator dye,the composition has an average temperature coefficient Δ
pH/Δ
T(Ta,Tb)≦
1×
10−
3 pH-unit/K for Ta=253 K and Tb=273 K, or Ta=273 K and Tb=293 K andthe HEPES or BisTrisPropane comprises 25-60 mole percent of the sum of the first and second buffers, and potassium phosphate comprises the remainder mole percent of the sum of the first and second buffers.
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Abstract
A method for preparing a composition that includes selecting a pH of the composition; selecting a first buffer with a negative temperature coefficient; selecting a second buffer with a positive temperature coefficient; and forming the composition comprising the first buffer and the second buffer. The composition has an average temperature coefficient, ΔpH/ΔT(Ta,Tb)≦1×10−3 pH-unit/K and a ΔpH(Ta,Tb)≦0.31 pH-unit for Ta=4 K and Tb=313 K.
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Citations
15 Claims
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1. A composition made by a method comprising:
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selecting a pH of the composition, the composition having a pH of 6 to 8; selecting a first buffer with a negative temperature coefficient, the first buffer comprising HEPES or BisTrisPropane; selecting a second buffer with a positive temperature coefficient, the second buffer comprising potassium phosphate; and forming the composition comprising the first buffer and the second buffer; wherein the composition does not comprise a pH-indicator dye, the composition has an average temperature coefficient Δ
pH/Δ
T(Ta,Tb)≦
1×
10−
3 pH-unit/K for Ta=253 K and Tb=273 K, or Ta=273 K and Tb=293 K andthe HEPES or BisTrisPropane comprises 25-60 mole percent of the sum of the first and second buffers, and potassium phosphate comprises the remainder mole percent of the sum of the first and second buffers. - View Dependent Claims (13, 15)
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2. A solution, comprising:
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a first buffer with a negative temperature coefficient, the first buffer comprising phthalate, acetate, MOPS, Tris, BisTris, BisTrisPropane, HEPES or MES; and a second buffer with a positive temperature coefficient, the second buffer comprising tartrate, phosphate, or borate; wherein the solution has an average temperature coefficient Δ
pH/Δ
T(Ta,Tb)≦
1×
10−
3 pH-unit/K for Ta=4 K and Tb=313 K, andwherein the solution does not comprise a pH-indicator dye. - View Dependent Claims (4, 5, 6, 7, 8)
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3. A buffer system, comprising:
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a first buffer with a positive temperature coefficient; and a second buffer with a negative temperature coefficient; wherein the buffer system has an average temperature coefficient Δ
pH/Δ
T(Ta,Tb)≦
1×
10−
3 pH-unit/K for Ta=4 K and Tb=313 K, a Δ
pH(4 K, 313 K)≦
0.31 pH-unit,and a buffering capacity of at least 0.01 for the temperature range of 4 K to 313 K, wherein the buffer system does not comprise a pH-indicator dye, and the buffer system has a buffer concentration of 25 mM to 75 mM. - View Dependent Claims (9, 10, 11, 12, 14)
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Specification