Triglyceride and cholesterol sensing method and sensor
First Claim
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1. An electrochemical method for the determination of the amount of triglyceride in a sample, said method comprising reacting the sample with a reagent mixture comprising(a2) a surfactant comprising one or more bile acid derivatives or salts thereof;
- (b2) a triglyceride hydrolysing reagent;
(c2) glycerol dehydrogenase;
(d2) a coenzyme; and
(e2) a redox agent capable of being oxidised or reduced to form a product;
and electrochemically detecting the amount of product formed.
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Abstract
A method for the determination of the amount of cholesterol in a sample is provided. The method typically provides a breakdown of the HDL and LDL cholesterol contents of the sample.
15 Citations
20 Claims
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1. An electrochemical method for the determination of the amount of triglyceride in a sample, said method comprising reacting the sample with a reagent mixture comprising
(a2) a surfactant comprising one or more bile acid derivatives or salts thereof; -
(b2) a triglyceride hydrolysing reagent; (c2) glycerol dehydrogenase; (d2) a coenzyme; and (e2) a redox agent capable of being oxidised or reduced to form a product; and electrochemically detecting the amount of product formed. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A reagent mixture for use in an electrochemical method for the determination of the amount of triglyceride in a sample, the reagent mixture comprising
(a2) a surfactant comprising one or more bile acid derivatives or salts thereof; -
(b2) a triglyceride hydrolysing reagent; (c2) glycerol dehydrogenase; (d2) a coenzyme; and (e2) a redox agent capable of being oxidised or reduced to form a product. - View Dependent Claims (12, 13, 14, 15)
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16. A kit for measuring the triglyceride content of a sample, the kit comprising
one or more electrochemical cells, each cell having a working electrode, a reference or pseudo reference electrode and optionally a separate counter electrode; -
a reagent mixture comprising; (a2) a surfactant comprising one or more bile acid derivatives or salts thereof; (b2) a triglyceride hydrolysing reagent; (c2) glycerol dehydrogenase; (d2) a coenzyme; and (e2) a redox agent capable of being oxidised or reduced to form a product, said reagent mixture being associated with one of said electrochemical cells; a power supply for applying a potential across each cell; and a measuring instrument for measuring the resulting electrochemical response of each cell. - View Dependent Claims (17, 18)
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19. A method of breaking down the lipoprotein structure of a sample comprising lipoproteins by using one or more bile acid derivatives or salts thereof as a surfactant, in an electrochemical method for the determination of the amount of triglyceride in a sample, said method comprising reacting the sample with a reagent mixture comprising
(a2) said one or more bile acid derivatives or salts thereof; -
(b2) a triglyceride hydrolysing reagent; (c2) glycerol dehydrogenase; (d2) a coenzyme; and (e2) a redox agent capable of being oxidised or reduced to form a product; and electrochemically detecting the amount of product formed.
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20. A method comprising:
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providing a liquid sample containing an amount of triglycerides, said triglycerides being bound to lipoproteins; providing a reagent mixture comprising (a2) a surfactant comprising one or more bile acid derivatives or salts thereof; (b2) a triglyceride hydrolysing reagent; (c2) glycerol dehydrogenase; (d2) a coenzyme; and (e2) a redox agent capable of being oxidised or reduced to form a product; freeing the triglycerides from the lipoproteins within the liquid sample by contacting the triglycerides with said surfactant comprising bile acid derivatives or salts thereof, said surfactant being configured to break the lipoproteins from the triglycerides; breaking down the freed triglycerides produced within the liquid sample into a proportionate amount of glycerol by reacting the freed triglycerides with said triglyceride hydrolyzing reagent; and electrochemically determining the amount of glycerol in the liquid sample by; reacting the glycerol with said glycerol dehydrogenase and said coenzyme in the presence of said redox agent; applying an electrical potential to the liquid sample; measuring the resulting electrochemical response; and correlating the measured response to the amount of glycerol; wherein the method provides an indication of the total triglyceride content of the liquid sample.
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Specification