Microphasic micro-components and methods for controlling morphology via electrified jetting

• US 8,187,708 B2
• Filed: 07/21/2009
• Issued: 05/29/2012
• Est. Priority Date: 11/10/2004
• Status: Active Grant

• Current Assignee: Board of Regents of the University of Michigan (University of Michigan)
• Original Assignee: Board of Regents of the University of Michigan (University of Michigan)
• Inventors: Lahann, Joerg, Kazemi, Abbass
• Primary Examiner(s): Mayes, Melvin
• Assistant Examiner(s): Qian, Yun
• Application Number:
  US12/506,712
• Publication Number:
  US 20100015447A1
• Time in Patent Office: 1,043 Days
• Field of Search: None
• US Class Current: 428/403
• CPC Class Codes: A61K 9/1676 having a drug-free core with discrete complete coating layer containing drug adsorbates of liquid drug formulations on inert powders without simultaneous granulation step A61K9/141; with further drug-free outer coating A61K9/5078; drug conjugated to non-active particles A61K47/6921
• CPC Class Codes: A61K 9/1694 resulting in granules or microspheres of the matrix type containing more than 5% of excipient
• CPC Class Codes: A61K 9/5026 obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyvinyl pyrrolidone, poly(meth)acrylates
• CPC Class Codes: A61K 9/5089 Processes
• CPC Class Codes: B01J 13/04 by physical processes, e.g. drying, spraying
• CPC Class Codes: B01J 13/14 Polymerisation; cross-linking
• CPC Class Codes: B01J 13/20 After-treatment of capsule walls, e.g. hardening
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: Y10T 428/2991 Coated

Abstract

The disclosure provides methods for preparation of multiphasic micro-components, such as core-shell or anisotropic (e.g., Janus) multiphasic particles with well-defined structures using electrohydrodynamic co-jetting of two polymer solutions containing polyelectrolytes. Suitable polyelectrolytes include polyacrylic acid (PAA), poly(acrylamide acrylic acid (PAAm), and/or poly(acryl amide-co-acrylic acid) (PAAm-AA), sodium polystyrene sulfonate (PSS), polyethylene imine (PEI), polypeptides, copolymers and combinations of these. Control of certain variables, such as relative conductivities of the two jetting solutions, controls the particle morphologies formed, leading to a predetermined phase orientation for the same polymer system. In certain aspects, after cross-linking, core-shell particles are stable in aqueous solutions and exhibit reproducible swelling behavior, while maintaining the original core-shell geometry. In addition, micro-components formed in accordance with the present teachings are pH-responsive based on external environmental pH. Thus, such micro-components are useful for a variety of different applications, including micro- and nano-active ingredient delivery systems.

Claims

1. A method of controlling the morphology of a multiphasic micro-component comprising:

providing a first liquid stream having a first electrical conductivity comprising a first polyelectrolyte and a second liquid stream having a second electrical conductivity comprising a second polyelectrolyte, wherein said first polyelectrolyte and said second polyelectrolyte are independently selected from the group consisting of;

polyacrylic acid (PAA), poly(acrylamide acrylic acid (PAAm), and/or poly(acryl amide-co-acrylic acid) (PAAm-AA), sodium polystyrene sulfonate (PSS), polyethylene imine (PEI), polypeptides, copolymers, and combinations thereof, wherein said first electrical conductivity and said second electrical conductivity are selected to create a predetermined phase orientation in a solid micro-component; and

exposing at least a portion of said first liquid stream and at least a portion of said second stream to an electric force field sufficient to form the solid micro-component comprising a first phase and a second distinct phase, wherein said first phase comprises material from said first liquid stream so that said first phase is hydrophilic and said second phase comprises material from said second liquid stream so that said second phase is hydrophilic, such that said first phase and said second phase are compositionally distinct from one another;

applying energy to cure said first polyelectrolyte and said second polyelectrolyte, so that the predetermined phase orientation in the solid micro-component is formed and the solid micro-component comprises a cross-linked first hydrophilic phase and a second cross-linked hydrophilic phase.

2. The method of claim 1, wherein a ratio of said first electrical conductivity to said second electrical conductivity is about 1:

1 to about 3;

1 and said predetermined phase orientation is anisotropic so that said first phase has a first exposed surface and said second phase has a second exposed surface.

3. The method of claim 1, wherein said exposing further comprises contacting a portion of an additional liquid stream with said first and second liquid streams, wherein said additional liquid stream is compositionally distinct from said first and second liquid streams and said exposing forms the solid micro-component comprising said first phase, said second phase, and an additional phase comprising a material from said additional liquid stream, wherein said first phase, said second phase, and said third phase are each compositionally distinct from one another.

4. The method of claim 1, wherein at least one of said first phase or said second phase comprises a component reactive to a pH of an external environment.

5. The method of claim 1, wherein a ratio of said first electrical conductivity to said second electrical conductivity is at least about 6:

1 and said predetermined phase orientation is a core-shell morphology.

6. The method of claim 1, wherein after said exposing, introducing said active ingredient into at least one of said first or second phases by contacting said active ingredient with a portion of said first or second phases for absorption therein.

7. A multiphasic micro-component comprising:

a first hydrophilic phase formed from a liquid stream comprising a first polyelectrolyte;

a second hydrophilic phase formed from a distinct liquid stream comprising a second polyelectrolyte;

wherein said first hydrophilic phase and said second hydrophilic phase are compositionally distinct from one another and are respectively cross-linked, wherein at least one of said first phase and said second phase comprises an active ingredient and said first phase has a first exposed surface and said second phase has a second exposed surface, so that said the multi-phasic micro-component has an anisotropic morphology.

8. The multiphasic micro-component of claim 7, wherein at least one of said first phase and said second phase undergoes reversible swelling in response to an external stimulus from a surrounding environment.

9. The multiphasic micro-component of claim 8, wherein said external stimulus is a pH of said surrounding environment.

10. The multiphasic micro-component of claim 7, wherein said active ingredient is selected from the group consisting of:

a therapeutic active ingredient, a systemic active ingredient, a chemotherapy active ingredient, a localized active ingredient, an oral care active ingredient, a nutritional active ingredient, a personal care active ingredient, a cosmetic active ingredient, a diagnostic imaging indicator agent, pesticides, growth-promoting agents, a home care active ingredient, an industrial or institutional active ingredient, and combinations thereof.

11. The multiphasic micro-component of claim 7, wherein said active ingredient is selected from the group consisting of:

low-molecular weight molecules, natural and artificial macromolecules, growth factors, growth factor receptors, transcriptional activators, translational promoters, anti-proliferative agents, growth hormones, anti-rejection drugs, anti-thrombotic agents, anti-coagulants, stem cell or gene therapy agents, antioxidants, free radical scavengers, nutrients, co-enzymes, ligands, peptides, proteins, nucleic acids, DNA, RNA, sugars, saccharides, nutrients, hormones, antibodies, immunomodulating agents, growth factor inhibitors, growth factor receptor antagonists, transcriptional repressors, translational repressors, replication inhibitors, inhibitory antibodies, cytotoxin, hormonal agonists, hormonal antagonists, inhibitors of hormone biosynthesis and processing, antigestagens, antiandrogens, anti-inflammatory agents, analgesics, non-steroidal anti-inflammatory agents (NSAIDs), COX-I and II inhibitors, antimicrobial agents, antiviral agents, antifungal agents, antibiotics, antineoplastic/antiproliferative/anti-miotic agents, anesthetic, analgesic or pain-killing agents, antipyretic agents, prostaglandin inhibitors, platelet inhibitors, DNA de-methylating agents, cholesterol-lowering agents, vasodilating agents, endogenous vasoactive interference agents, angiogenic substances, cardiac failure active ingredients, polysaccharides, sugars, targeting toxin agents, aptamers, quantum dots, nano-materials, nano-crystals, localized drugs, tooth whitening agents, skin whitening agents, anti-caries agents, anti-tartar agents, anti-plaque agents, anti-adhesion agents, desensitizing agents, malodor control agents, flavoring agents, anti-aging agents, salivary stimulants, periodontal actives, depigmentation agents, skin lightening agents, reflectants, humectants, allergy inhibitors, anti-acne agents, anti-aging agents, anti-wrinkling agents, antiseptics, keratolytic agents, fresheners, healing agents, inflammation inhibitors, wound healing promoters, deodorants, antiperspirants, skin emollients, tanning agents, antifungals, depilating agents, counterirritants, non-steroidal soothing agents, anti-itch agents, poison ivy agents, poison oak agents, burn alleviation agents, vitamins, cooling agents, heating agents, chelating agents, anti-psoriasis agents, anti-dandruff agents, skin conditioners, moisturizing agents, emollients, humectants, occlusive agents, skin lipid fluidizers, deodorant active agents, antiperspirant active agents, skin and/or scalp sensates, skin and/or scalp soothing and/or healing agents, astringents, opacifying agents, biocides, natural and synthetic extracts and essential oils, nutrients, enzymes, proteins, amino acids, vitamins, analgesics, sunscreen agents, UV absorbers, antioxidants, antibiotics, exfoliants, cell turnover enhancers, coloring agents, sunscreens, nourishing agents, moisture absorbers, sebum absorbers, skin penetration enhancers, colorants, pigments, dyes, flavors, fragrances, detergents, fabric softeners, hard surface cleaning agents, bleaching agents, surface active agents, phosphates, builders, anti-deposition agents, imaging or contrast agents, pesticides, growth-promoting agents, and combinations thereof.

12. A multiphasic core-shell micro-component comprising:

a first cross-linked phase defining a core region formed from a liquid stream comprising a first polyelectrolyte that comprises polyacrylic acid (PAA);

a second cross-linked phase defining a shell region externally surrounding at least a portion of said core region formed from a distinct liquid stream comprising a second polyelectrolyte that comprises poly(acryl amide-co-acrylic acid) (PAAm-AA);

wherein said first phase and said second phase are compositionally distinct from one another, wherein at least one of said first phase and said second phase comprises an active ingredient.

13. The multiphasic core-shell micro-component of claim 12, wherein at least one of said first phase and said second phase undergoes reversible swelling in response to an external stimulus from a surrounding environment.

14. The multiphasic core-shell micro-component of claim 12, wherein at least one of said first phase and said second phase reacts to an external stimulus selected from the group consisting of pH, humidity, pressure, light, temperature, applied energy fields, electrical fields, magnetic fields, or combinations thereof.

15. The multiphasic core-shell micro-component of claim 12, wherein said first cross-linked phase or said second cross-linked phase further comprises an additional polymer distinct from said first polyelectrolyte or said second polyelectrolyte, the additional polymer selected from the group consisting of:

polyacrylic acid (PAA), poly(acrylamide acrylic acid (PAAm), and/or poly(acryl amide-co-acrylic acid) (PAAm-AA), sodium polystyrene sulfonate (PSS), polyethylene imine (PEI), polypeptides, copolymers and combinations thereof.

16. The multiphasic core-shell micro-component of claim 12, wherein the micro-component is a capsule for delivery of an active agent selected from the group consisting of:

a therapeutic active ingredient, a systemic active ingredient, a chemotherapy active ingredient, a localized active ingredient, an oral care active ingredient, a nutritional active ingredient, a personal care active ingredient, a cosmetic active ingredient, a diagnostic imaging indicator agent, pesticides, growth-promoting agents, a home care active ingredient, an industrial or institutional active ingredient, and combinations thereof.

17. The multiphasic core-shell micro-component of claim 12, wherein a conductivity mismatch (Δ

σ

) between a first conductivity of the liquid stream comprising the first polyelectrolyte and a second conductivity of the distinct liquid stream comprising the second polyelectrolyte is greater than or equal to about 200%.

18. The multiphasic core-shell micro-component of claim 12, wherein said first cross-linked phase further comprises poly(acryl amide-co-acrylic acid) (PAAm-AA) in addition to said first polyelectrolyte comprising said polyacrylic acid (PAA) and said second cross-linked phase further comprises polyacrylic acid (PAA) in addition to said second polyelectrolyte comprising said poly(acryl amide-co-acrylic acid) (PAAm-AA).

19. A multiphasic micro-component comprising:

a first hydrophilic phase formed from a liquid stream comprising a first polyelectrolyte;

a second hydrophilic phase formed from a distinct liquid stream comprising a second polyelectrolyte;

wherein said first polyelectrolyte and said second polyelectrolyte are independently selected from the group consisting of;

polyacrylic acid (PAA), poly(acrylamide acrylic acid (PAAm), and/or poly(acryl amide-co-acrylic acid) (PAAm-AA), sodium polystyrene sulfonate (PSS), polyethylene imine (PEI), polypeptides, copolymers and combinations thereof and wherein said first hydrophilic phase and said second hydrophilic phase are compositionally distinct from one another and are respectively cross-linked, wherein at least one of said first phase and said second phase comprises an active ingredient.

20. The multiphasic micro-component of claim 19, wherein said first polyelectrolyte polymer comprises polyacrylic acid (PAA) and said second polyelectrolyte polymer comprises poly(acryl amide-co-acrylic acid) (PAAm-AA).

21. The multiphasic micro-component of claim 20, wherein said first phase further comprises poly(acryl amide-co-acrylic acid) (PAAm-AA) in addition to said first polyelectrolyte polymer comprising said polyacrylic acid (PAA) and said second phase further comprises polyacrylic acid (PAA) in addition to said second polyelectrolyte polymer comprising said poly(acryl amide-co-acrylic acid) (PAAm-AA).