Methods related to wound healing

• US 8,187,881 B2
• Filed: 12/01/2009
• Issued: 05/29/2012
• Est. Priority Date: 03/29/2006
• Status: Active Grant

• Current Assignee: Stemnion, Inc.
• Original Assignee: Stemnion, Inc.
• Inventors: Smith, Charlotte A., Trumpower, Catherine J., Marshall, Vivienne S.
• Primary Examiner(s): Driscoll, Lora E Barnhart
• Application Number:
  US12/592,658
• Publication Number:
  US 20100080779A1
• Time in Patent Office: 910 Days
• Field of Search: None
• US Class Current: 435/405
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61K 35/50 Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
• CPC Class Codes: A61P 17/02 for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: C12N 2501/11 Epidermal growth factor [EGF]
• CPC Class Codes: C12N 2502/02 embryonic cells
• CPC Class Codes: C12N 5/0605 Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
• CPC Class Codes: C12N 5/0629 Keratinocytes; Whole skin

Abstract

The invention is directed to methods for the treatment of wounds. Such methods utilize novel compositions, including but not limited to amnion-derived multipotent cells (herein referred to as AMP cells), conditioned media derived therefrom (herein referred to as amnion-derived cellular cytokine suspension or ACCS), cell lysates derived therefrom, cell products derived therefrom, each alone or in combination.

Claims

1. A method for accelerating wound healing of a treated burn wound as compared to the healing rate of a non-treated burn wound in a patient in need thereof comprising treating the burn wound by topically administering to the patient'"'"'s burn wound 0.50 μ

L-2000 μ

L conditioned medium per cm² of burn wound, wherein the conditioned medium is made by

a) obtaining a placenta and isolating an amnion from the placenta;

b) enzymatically releasing amnion-derived epithelial cells from the amnion;

c) collecting the released amnion-derived epithelial cells;

d) culturing the collected amnion-derived epithelial cells of step (c) in basal culture medium that is supplemented with human serum albumin and human recombinant EGF; and

e) collecting the conditioned medium.

2. The method of claim 1 wherein the treatment increases tensile strength and breaking strength of the healing treated burn wound as compared to the tensile strength and breaking strength in a healing non-treated burn wound.

3. The method of claim 1 wherein the treatment reduces fibrosis and collagen contraction in a healing treated burn wound as compared to the fibrosis and collagen contraction in a healing non-treated burn wound.

4. The method of claim 1 wherein the conditioned medium is pooled conditioned medium.