Oligonucleotides for amplifying Chlamydophila pneumoniae nucleic acid

• US 8,188,258 B2
• Filed: 12/16/2010
• Issued: 05/29/2012
• Est. Priority Date: 06/06/2005
• Status: Active Grant

• Current Assignee: Gen-Probe Incorporated (Hologic Incorporated)
• Original Assignee: Gen-Probe Incorporated (Hologic Incorporated)
• Inventors: Cunningham, Melissa M.
• Primary Examiner(s): Kim, Young J
• Application Number:
  US12/969,858
• Publication Number:
  US 20110091894A1
• Time in Patent Office: 530 Days
• Field of Search: None
• US Class Current: 536/24.33
• CPC Class Codes: C12Q 1/689 for bacteria

Abstract

Oligonucleotides for use in determining the presence of Chlamydophila pneumoniae in a test sample. The oligonucleotides are incorporated into detection probes, capture probes, and amplification oligonucleotides, and can be used in various combinations thereof.

Claims

1. A kit for amplifying a target sequence present in a target nucleic acid derived from Chlamydophila pneumonia, the kit comprising in packaged combination:

a first amplification oligonucleotide, the base sequence of which consists of (i) a target binding region that is fully complementary to a base sequence selected from the group consisting of SEQ ID NO;

48, SEQ ID NO;

56, and SEQ ID NO;

60, and, optionally, (ii) a 5′

promoter sequence; and

a second amplification oligonucleotide, the base sequence of which consists of (i) a target binding region that is fully complementary to the base sequence of SEQ ID NO;

83, and, optionally, (ii) a 5′

promoter sequence.

2. The kit of claim 1, wherein the first amplification oligonucleotide consists of the target binding region and the 5′

promoter sequence.