Signal enhancement using a switchable magnetic trap
First Claim
1. An apparatus for analysis of a sample, comprising:
- a microchip,a microchannel flow channel in said microchip,a connecting channel connected to said microchannel flow channel,a source of magnetic particles that produces magnetic particles, said source of magnetic particles connected to said connecting channel wherein said source of magnetic particles delivers said magnetic particles to said connecting channel,a source of carrier fluid connected to said connecting channel for moving the sample in said connecting channel,a source of the sample connected to said connecting channel,fluorescent probes associated with said magnetic particles, said carrier fluid, and said sample,a source of microchannel flow channel carrier fluid connected to said microchannel flow channel wherein said microchannel flow channel carrier fluid is positioned to move the sample, said magnetic particles, and said fluorescent probes from said connecting channel into said microchannel flow channel and to move the sample, said magnetic particles, and said fluorescent probes in the microchannel flow channel,an electromagnet trap connected to said microchannel flow channel for selectively magnetically trapping the sample, said magnetic particles, and said fluorescent probes, andan analyzer positioned at or proximate said electromagnet trap for detecting said fluorescent probes and analyzing the sample.
3 Assignments
0 Petitions
Accused Products
Abstract
A system for analyzing a sample including providing a microchannel flow channel; associating the sample with magnetic nanoparticles or magnetic polystyrene-coated beads; moving the sample with said magnetic nanoparticles or magnetic polystyrene-coated beads in the microchannel flow channel; holding the sample with the magnetic nanoparticles or magnetic polystyrene-coated beads in a magnetic trap in the microchannel flow channel; and analyzing the sample obtaining an enhanced analysis signal. An apparatus for analysis of a sample includes magnetic particles connected to the sample, a microchip, a flow channel in the microchip, a source of carrier fluid connected to the flow channel for moving the sample in the flow channel, an electromagnet trap connected to the flow line for selectively magnetically trapping the sample and the magnetic particles, and an analyzer for analyzing the sample.
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Citations
27 Claims
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1. An apparatus for analysis of a sample, comprising:
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a microchip, a microchannel flow channel in said microchip, a connecting channel connected to said microchannel flow channel, a source of magnetic particles that produces magnetic particles, said source of magnetic particles connected to said connecting channel wherein said source of magnetic particles delivers said magnetic particles to said connecting channel, a source of carrier fluid connected to said connecting channel for moving the sample in said connecting channel, a source of the sample connected to said connecting channel, fluorescent probes associated with said magnetic particles, said carrier fluid, and said sample, a source of microchannel flow channel carrier fluid connected to said microchannel flow channel wherein said microchannel flow channel carrier fluid is positioned to move the sample, said magnetic particles, and said fluorescent probes from said connecting channel into said microchannel flow channel and to move the sample, said magnetic particles, and said fluorescent probes in the microchannel flow channel, an electromagnet trap connected to said microchannel flow channel for selectively magnetically trapping the sample, said magnetic particles, and said fluorescent probes, and an analyzer positioned at or proximate said electromagnet trap for detecting said fluorescent probes and analyzing the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. An apparatus for analysis of a sample comprising:
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a microchip, a microchannel flow channel in said microchip, a connecting channel connected to said microchannel flow channel, a source of magnetic particles connected to said connecting channel that delivers said magnetic particles to said connecting channel, a source of carrier fluid connected to said connecting channel for moving the sample in said connecting channel, a source of the sample connected to said connecting channel, fluorescence probes associated with said magnetic particles, said carrier fluid, and said sample, a pulsed source of carrier fluid connected to said flow channel that pulses carrier fluid into said flow channel and creates liquid slugs in said flow channel containing the sample, said magnetic particles, and said fluorescent probes, an electromagnet trap connected to said flow channel for selectively magnetically trapping said liquid slugs containing the sample, said magnetic particles, and said fluorescent probes, and an analyzer positioned at or proximate said electromagnet trap for detecting said fluorescent probes and analyzing the sample.
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16. An apparatus for analysis of a sample, comprising:
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a microchip, a microchannel flow channel in said microchip, a connecting channel connected to said microchannel flow channel, a source of magnetic particles connected to said connecting channel that delivers said magnetic particles to said connecting channel, a source of carrier fluid connected to said connecting channel for moving the sample in said connecting channel, a source of the sample connected to said connecting channel, fluorescent probes associated with said magnetic articles said carrier fluid, and said sample, a droplet maker connected to said flow channel for producing droplets containing the sample, said fluorescent probes, and said magnetic particles, an electromagnet trap connected to said flow channel for selectively magnetically trapping said droplets containing the sample, said fluorescent probes, and said magnetic particles, and an analyzer positioned at or proximate said electromagnet trap for detecting said fluorescent probes and analyzing the sample. - View Dependent Claims (17)
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18. A method of analyzing a sample on a microchip, comprising the steps of:
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providing a microchannel flow channel in the microchip; providing a connecting channel connected to said microchannel flow channel, providing a source of carrier fluid connected to said connecting channel for moving the sample in said connecting channel, providing a source of the sample connected to said connecting channel, providing a source of magnetic nanoparticles or magnetic polystyrene-coated beads connected to said connecting channel, providing fluorescent probes associated with the sample, with said carrier fluid, and with said magnetic nanoparticles or magnetic polystyrene-coated beads; moving the sample with said fluorescent probes and said magnetic nanoparticles or magnetic polystyrene-coated beads in said connecting channel into said microchannel flow channel and in said microchannel flow channel, trapping the sample with said fluorescent probes and said magnetic nanoparticles or magnetic polystyrene-coated beads in a magnetic trap in said microchannel flow channel, and providing an analyzer positioned at or proximate said electromagnet trap for detecting said fluorescent probes and analyzing the sample. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25)
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26. A method of analyzing a sample, comprising the steps of:
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providing a microchannel flow channel; providing a connecting channel connected to said microchannel flow channel, providing a source of carrier fluid connected to said connecting channel for moving the sample in said connecting channel, providing a source of the sample connected to said connecting channel, providing a source of magnetic nanoparticles or magnetic polystyrene-coated beads connected to said connecting channel, providing fluorescent probes associated with the sample, with said carrier fluid, and with said magnetic nanoparticles or magnetic polystyrene-coated beads; providing a droplet maker connected to said connecting channel and to said flow channel for producing droplets containing the sample, said fluorescent probes, and said magnetic nanoparticles or magnetic polystyrene-coated beads, moving said droplets with the sample, with said fluorescent probes, and with said magnetic nanoparticles or magnetic polystyrene-coated beads in said microchannel flow channel; holding said droplets with the sample, with said fluorescent probes, and with said magnetic nanoparticles or magnetic polystyrene-coated beads in a magnetic trap in said microchannel flow channel; and providing an analyzer positioned at or proximate said electromagnet trap for detecting said fluorescent probes and analyzing the sample obtaining an enhanced analysis signal. - View Dependent Claims (27)
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Specification