Biochemical concentrator and drug discovery

• US 8,192,966 B2
• Filed: 05/15/2009
• Issued: 06/05/2012
• Est. Priority Date: 03/18/2004
• Status: Expired due to Fees

• Current Assignee: University of Hawai'i
• Original Assignee: University of Hawai'i
• Inventors: Holm-Kennedy, James W.
• Primary Examiner(s): Yang, N. C.
• Application Number:
  US12/454,295
• Publication Number:
  US 20100122906A1
• Time in Patent Office: 1,117 Days
• Field of Search: None
• US Class Current: 435/149
• CPC Class Codes: G01N 27/3276 being a hybridisation with immobilised receptors using a FET type sensor G01N27/4145; concerning the hybridisation C12Q1/68

Abstract

A novel label-free sensitive detection method by employing a novel sensitive charge sensor is provided. Dissociation constant information is provided by a simple measurement of the dissociation of the target molecule form the target'"'"'s receptor. The later process is affected by a novel system and its configuration as described herein. Basic objectives are to provide a drug discovery and characterization system that is an improvement over the current state of the art, low cost, highly sensitive, accurate, fast and easy to use. This invention involves both a physical system and a methodology.

Claims

1. A method of concentrating target species in a solution comprising:

providing a solution,

providing species in the solution,

providing receptors for receiving the target species on the sensor,

providing an electric field in the solution,

influencing movement of the target species toward the sensor with the electric field, and

wherein the providing the electric field comprises providing at least one electrode grid in the solution surrounding the sensor and connecting at least one voltage source between the at least one electrode and the sensor.

2. The method of claim 1, wherein the providing the at least one electrode further comprises providing plural grids in the solution spaced from each other and from the sensor and wherein the connecting the at least one voltage source comprises connecting voltage sources between the sensor and a grid and connecting voltage sources between grids.

3. The method of claim 2, wherein the grids are differentially shaped from each other and are differentially spaced from each other.

4. The method of claim 2, further comprising providing a varying or variable electric field, steady pulsating, alternating, sinusoidally alternating, or other DC or AC voltage to the grids.

5. A method of drug discovery comprising providing a solution, providing target drug species in the solution, wherein the target drug species is charged naturally or by attaching a charge bearing particle to the target drug species to result in a net charge, providing an electrode grid in the solution surrounding a sensor, providing an electric field in the solution and moving the target drug species toward the sensor under influence of the electric field.

6. The method of claim 5, wherein the providing an electric field further comprises providing multiple electric fields in the solution and wherein the providing and electrode further comprises providing multiple spaced grids in the solution and connecting multiple voltage sources between the spaced grids.

7. The method of claim 6 further comprising providing voltages between the spaced grids.

8. The method of claim 6, further comprising bonding the target species with receptors on the sensor and measuring the target species in contact with the receptors.

9. The method of claim 8, further comprising reversing the field and tending to move the target species away from the sensor and measuring the decay of target species in contact with the receptors.

10. The method of claim 9, further comprising eluating the target species from the sensor and measuring and determining the target species in a resultant eluent.

11. The method of claim 10, wherein the providing target drug species comprises providing plural target drug species and wherein the method further comprises simultaneously evaluating and comparing the plural target drug species.

12. The method of claim 5, further comprising attaching a charge bearing particle to the target drug species.

13. A method of drug discovery comprising:

providing a solution containing a target species;

providing one or more electric field generators;

generating electric fields and effecting movement of the target species within the solution;

providing a sensor having receptors for the target species;

providing one or more voltage sources;

connecting the one or more voltage sources to the one or more electric field generators;

providing a ground connected to the sensor and to the one or more sources;

wherein the providing the one or more electric field generators, the generating the electric field, the providing one or more voltage sources and the connecting the one or more voltage sources to the one or more electric field generators and providing a ground connected to the sensor and to the one or more sources comprises providing a bioconcentrator;

concentrating the target species in the region of the solution nearest the sensor;

removing the target species from that region and from the vicinity of one or more electric field generators, without necessarily applying an electric field in the area around the sensor during operation;

wherein the providing of one or more electric field generators further comprises providing one or more electrode grids spaced from the sensor;

wherein the providing of one or more electrodes further comprises providing one or more grids surrounding the sensor and spacing the grids between the sensor and each respective electrode'"'"'s voltage source.

14. The method of claim 13, further comprising providing multiple grids surrounding the sensor and providing multiple voltage sources connected between the grids.

15. The method of claim 14, wherein the multiple grids are shaped for effecting concentrations of the species near the sensor.

16. The method of claim 14, wherein the source provides pulsed, alternating, sinusoidal or other DC or AC power to the field generator.