Composite scaffolds and methods using same for generating complex tissue grafts
First Claim
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1. A method of forming a heterogeneous tissue, the method comprising:
- (a) growing vascular cells on a filamentous scaffold so as to form tubular vascular structures thereupon;
(b) embedding said filamentous scaffold comprising said tubular vascular structures in a porous continuous scaffold thereby forming a composite scaffold;
(c) contacting said composite scaffold with cells of a non-vascular tissue type; and
(d) growing said vascular cells and said cells of a non-vascular type in said composite scaffold, thereby forming said heterogenous tissue.
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Abstract
A composite scaffold for engineering a heterogeneous tissue is provided. The composite scaffold includes: (a) a first scaffold being capable of supporting formation of a first tissue type thereupon; and (b) a second scaffold being capable of supporting formation of a second tissue type thereupon; wherein the first scaffold and the second scaffold are arranged with respect to each other such that when the first scaffold supports the first tissue type and the second scaffold supports the second tissue type, a distance between any cell of the second tissue type and the first tissue type does not exceed 200 μm.
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18 Claims
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1. A method of forming a heterogeneous tissue, the method comprising:
- (a) growing vascular cells on a filamentous scaffold so as to form tubular vascular structures thereupon;
(b) embedding said filamentous scaffold comprising said tubular vascular structures in a porous continuous scaffold thereby forming a composite scaffold;
(c) contacting said composite scaffold with cells of a non-vascular tissue type; and
(d) growing said vascular cells and said cells of a non-vascular type in said composite scaffold, thereby forming said heterogenous tissue. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
- (a) growing vascular cells on a filamentous scaffold so as to form tubular vascular structures thereupon;
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