Substrate for biochip, biochip, method for manufacturing substrate for biochip, and method for manufacturing biochip

• US 8,198,071 B2
• Filed: 04/04/2011
• Issued: 06/12/2012
• Est. Priority Date: 09/16/2005
• Status: Expired due to Fees

• Current Assignee: Azbil Corporation (Azbil BioVigilant Incorporated)
• Original Assignee: Azbil Corporation (Azbil BioVigilant Incorporated)
• Inventors: Goshoo, Yasuhiro, Kuroiwa, Takaaki, Ishikawa, Naohiro, Obara, Daisuke, Yamasaki, Shinsuke, Sasaki, Kazuko, Horiguchi, Yasuko
• Primary Examiner(s): FORMAN, BETTY J
• Application Number:
  US13/079,483
• Publication Number:
  US 20110174773A1
• Time in Patent Office: 435 Days
• Field of Search: None
• US Class Current: 435/283.1
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00675 In-situ synthesis on the substrate
• CPC Class Codes: B01J 2219/00729 Peptide nucleic acids [PNA]
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: G01N 33/54353 with ligand attached to the carrier via a chemical coupling agent coatings G01N33/54393
• CPC Class Codes: G01N 33/54386 Analytical elements
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
• CPC Class Codes: Y10T 428/24331 including nonapertured component
• CPC Class Codes: Y10T 428/31525 Next to glass or quartz
• CPC Class Codes: Y10T 428/31529 Next to metal

Abstract

A base plate having a surface on which a plurality of hydroxyl groups can be introduced, a metallic membrane disposed on the base plate and having a plurality of wells reaching the base plate, and a crosslinkable polymer membrane disposed on the metallic membrane are included.

Claims

1. A method for manufacturing a biochip including:

preparing a base plate;

forming a metallic membrane on the base plate;

forming a crosslinkable polymer membrane on the metallic membrane;

selectively removing portions of the polymer membrane;

delineating a plurality of wells reaching the base plate in the metallic membrane by using the polymer membrane as an etching mask;

introducing a plurality of hydroxyl groups on a surface of the base plate exposed from the plurality of wells;

bonding a plurality of probe biomolecules including protected amino groups to the plurality of hydroxyl groups; and

soaking the base plate, the metallic membrane and the polymer membrane in an alkaline solution to deprotect the amino groups included in the plurality of probe biomolecules and peel off the polymer membrane from the metallic membrane after the probe biomolecules are introduced on the base plate,

wherein the crosslinkable polymer membrane protects the metallic membrane while the probe biomolecules are introduced on the plate exposed from the plurality of wells.

2. The method for manufacturing the biochip of claim 1, wherein the base plate is composed of a silicon oxide.

3. The method for manufacturing the biochip of claim 1, wherein the metallic membrane is composed of titanium.

4. The method for manufacturing the biochip of claim 1, wherein the metallic membrane is composed of a transition metallic oxide.

5. The method for manufacturing the biochip of claim 1, wherein the metallic membrane is composed of a transition metal nitride.

6. The method for manufacturing the biochip of claim 1, wherein the metallic membrane is composed of a transition metal carbide.

7. The method for manufacturing the biochip of claim 1, wherein the polymer membrane is composed of an epoxy resin.

8. The method for manufacturing the biochip of claim 1, wherein the step for peeling off the polymer membrane from the metallic membrane includes a step for blowing the polymer membrane with an air.

9. A method for manufacturing a biochip including:

preparing a substrate for the biochip comprising a base plate, a metallic membrane disposed on the base plate and having a plurality of wells reaching the base plate, a crosslinkable polymer membrane disposed on the metallic membrane, and a plurality of hydroxyl groups introduced on a surface of the base plate exposed from the plurality of wells;

bonding a plurality of probe biomolecules including protected amino groups to the plurality of hydroxyl groups; and

soaking the base plate, the metallic membrane and the polymer membrane in an alkaline solution to deprotect amino groups included in the plurality of probe biomolecules and peel off the polymer membrane from the metallic membrane after the probe biomolecules are introduced on the base plate,

wherein the crosslinkable polymer membrane protects the metallic membrane while the probe biomolecules are introduced on the plate exposed from the plurality of wells.

10. The method for manufacturing the biochip of claim 9, wherein the metallic membrane is composed of titanium.

11. The method for manufacturing the biochip of claim 9, wherein the metallic membrane is composed of a transition metallic oxide.

12. The method for manufacturing the biochip of claim 9, wherein the metallic membrane is composed of a transition metal nitride.

13. The method for manufacturing the biochip of claim 9, wherein the metallic membrane is composed of transition metal carbide.

14. The method for manufacturing the biochip of claim 9, wherein the polymer membrane is composed of an epoxy resin.