Membranes and methods for coating membranes
First Claim
1. A microporous, absorbent, blood-separating membrane having opposed surfaces, a reactive film coated on one of said membrane surfaces, wherein the reactive film is a water-resistant, water-insoluble film which contains(a) all or at least portions of a detection reagent for an analyte to be detected in a hydrophobic matrix of one or more film formers and(b) film openers comprising inorganic or organic particulate, water insoluble, non-swelling wettable fillers which do not substantially scatter light and which enable large molecules and cells to penetrate into said reactive film wherein the mass ratio of film opener to film former is about 10:
- 1 to about 1;
1,wherein said microporous, absorbent, blood-separating membrane is substantially free of detection reagents for an analyte.
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Accused Products
Abstract
A method for applying reactive films containing solids to microporous membranes is provided wherein the membrane is firstly moistened and the reactive film containing solids is applied to the membrane while it is still moist. Membranes produced in this manner can contain reactive films having a high proportion of film openers and can be used advantageously in diagnostic elements for detecting constituents and, in particular, large hydrophobic analytes in body fluids.
20 Citations
13 Claims
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1. A microporous, absorbent, blood-separating membrane having opposed surfaces, a reactive film coated on one of said membrane surfaces, wherein the reactive film is a water-resistant, water-insoluble film which contains
(a) all or at least portions of a detection reagent for an analyte to be detected in a hydrophobic matrix of one or more film formers and (b) film openers comprising inorganic or organic particulate, water insoluble, non-swelling wettable fillers which do not substantially scatter light and which enable large molecules and cells to penetrate into said reactive film wherein the mass ratio of film opener to film former is about 10: - 1 to about 1;
1,wherein said microporous, absorbent, blood-separating membrane is substantially free of detection reagents for an analyte. - View Dependent Claims (2, 3, 4, 5, 6, 7)
- 1 to about 1;
-
8. A composite structure for determining the presence of an analyte in a sample of blood, said composite structure comprising
(a) a microporous absorbent, blood-separating membrane having opposed first and second surfaces, said membrane being operable to separate plasma or serum from whole blood, and (b) a water-resistant, water-insoluble reactive film coated on one of said opposed surfaces of said membrane, said reactive film comprising (i) a hydrophobic film matrix of one or more film formers, said matrix containing a detection reagent for an analyte to be determined in said blood sample, and (ii) film openers comprising inorganic or organic particulate, water insoluble, non-swelling wettable fillers which do not substantially scatter light and which enable large molecules and cells to penetrate into said reactive film wherein the mass ratio of film opener to film former is about 10: - 1 to about 1;
1. - View Dependent Claims (9, 10, 11, 12, 13)
- 1 to about 1;
Specification