Enzyme compositions and methods for the improved enzymatic hydrolysis of cellulose
First Claim
1. A process for the enzymatic hydrolysis of cellulose to produce a hydrolysis product comprising glucose from a pretreated lignocellulosic feedstock, the process comprising:
- (i) partially hydrolyzing an aqueous slurry of the pretreated lignocellulosic feedstock with an enzyme composition to produce a hydrolyzed slurry comprising glucose, glucose oligomers or a combination thereof, and unhydrolyzed fiber solids comprising cellulose and lignin, said enzyme composition comprising cellulase enzymes and at least one β
-glucosidase enzyme comprising a cellulase binding domain that binds said β
-glucosidase enzyme to said pretreated lignocellulosic feedstock;
(ii) separating the unhydrolyzed fiber solids from the aqueous phase to produce separated fiber solids, wherein the cellulase enzymes and the at least one β
-glucosidase enzyme comprising a cellulose binding domain bind to the unhydrolyzed separated fiber solids;
(iii) re-suspending the unhydrolyzed separated fiber solids in an aqueous solution to produce a re-suspended slurry; and
(iv) continuing the hydrolysis of the re-suspended slurry to produce the hydrolysis product comprising glucose.
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Abstract
A process for the enzymatic hydrolysis of cellulose to produce a hydrolysis product comprising glucose from a pretreated lignocellulosic feedstock and enzymes for use in the process are provided. The process comprises partially hydrolyzing an aqueous slurry of a pretreated lignocellulosic feedstock with cellulase enzymes, one or more than one β-glucosidase enzyme and a binding agent for binding the β-glucosidase enzyme to fiber solids present in the aqueous slurry. The unhydrolyzed fiber solids are then separated from the hydrolyzed slurry. The separated fiber solids thus obtained are then re-suspended in an aqueous solution to produce a re-suspended slurry. The hydrolysis is then continued to produce the hydrolysis product comprising glucose.
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Citations
22 Claims
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1. A process for the enzymatic hydrolysis of cellulose to produce a hydrolysis product comprising glucose from a pretreated lignocellulosic feedstock, the process comprising:
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(i) partially hydrolyzing an aqueous slurry of the pretreated lignocellulosic feedstock with an enzyme composition to produce a hydrolyzed slurry comprising glucose, glucose oligomers or a combination thereof, and unhydrolyzed fiber solids comprising cellulose and lignin, said enzyme composition comprising cellulase enzymes and at least one β
-glucosidase enzyme comprising a cellulase binding domain that binds said β
-glucosidase enzyme to said pretreated lignocellulosic feedstock;(ii) separating the unhydrolyzed fiber solids from the aqueous phase to produce separated fiber solids, wherein the cellulase enzymes and the at least one β
-glucosidase enzyme comprising a cellulose binding domain bind to the unhydrolyzed separated fiber solids;(iii) re-suspending the unhydrolyzed separated fiber solids in an aqueous solution to produce a re-suspended slurry; and (iv) continuing the hydrolysis of the re-suspended slurry to produce the hydrolysis product comprising glucose. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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Specification
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- Resources
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Current AssigneeIogen Energy Corporation (Iogen Corporation)
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Original AssigneeIogen Energy Corporation (Iogen Corporation)
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InventorsTolan, Jeffrey, White, Theresa, Tomashek, John
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Primary Examiner(s)RAGHU, GANAPATHIRAM
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Application NumberUS12/305,069Publication NumberTime in Patent Office1,824 DaysField of Search435/99, 435/209, 435/200, 435/262, 435/274US Class Current435/99CPC Class CodesC12N 9/2437 Cellulases (3.2.1.4; 3.2.1....C12N 9/2445 Beta-glucosidase (3.2.1.21)C12P 19/02 Monosaccharides 2-ketogulon...C12P 7/10 substrate containing cellul...C12Y 302/01021 Beta-glucosidase (3.2.1.21)Y02E 50/10 Biofuels, e.g. bio-dieselY02P 20/582 Recycling of unreacted star...
