Compositions for detecting group A streptococci
First Claim
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1. A composition for amplifying in a transcription associated amplification reaction and detecting in real time a Streptococcus pyogenes target nucleic acid sequence, said composition comprising:
- a first primer, the base sequence of which consists of a 3′
terminal target-complementary sequence and, optionally, a first primer upstream promoter sequence that is non-complementary to a nucleic acid containing said target nucleic acid sequence or its complement, said 3′
terminal target-complementary sequence of said first primer being selected from the group consisting of SEQ ID NO;
9, SEQ ID NO;
10, SEQ ID NO;
11, SEQ ID NO;
12 and SEQ ID NO;
13, allowing for the presence of RNA equivalents and nucleotide analogs;
a second primer, the base sequence of which consists of a 3′
terminal target-complementary sequence and, optionally, a second primer upstream promoter sequence that is non-complementary to a nucleic acid containing said target nucleic acid sequence or its complement, said 3′
terminal target-complementary sequence of said second primer being selected from the group consisting of SEQ ID NO;
4, SEQ ID NO;
5, SEQ ID NO;
6, and SEQ ID NO;
7, allowing for the presence of RNA equivalents and nucleotide analogs; and
a molecular beacon probe, the base sequence of which consists of a target-complementary sequence and a pair of mutually complementary base sequences that are non-complementary to a nucleic acid containing said target nucleic acid sequence or its complement, said target-complementary sequence of said probe being selected from the group consisting of SEQ ID NO;
23, SEQ ID NO;
24 and SEQ ID NO;
25, allowing for the presence of DNA equivalents and nucleotide analogs.
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Abstract
Compositions, methods and kits for detecting Group A streptococci. Particularly described are oligonucleotides that are useful as amplification primers and hybridization probes for detecting very low levels of Group A streptococci nucleic acids.
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Citations
12 Claims
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1. A composition for amplifying in a transcription associated amplification reaction and detecting in real time a Streptococcus pyogenes target nucleic acid sequence, said composition comprising:
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a first primer, the base sequence of which consists of a 3′
terminal target-complementary sequence and, optionally, a first primer upstream promoter sequence that is non-complementary to a nucleic acid containing said target nucleic acid sequence or its complement, said 3′
terminal target-complementary sequence of said first primer being selected from the group consisting of SEQ ID NO;
9, SEQ ID NO;
10, SEQ ID NO;
11, SEQ ID NO;
12 and SEQ ID NO;
13, allowing for the presence of RNA equivalents and nucleotide analogs;a second primer, the base sequence of which consists of a 3′
terminal target-complementary sequence and, optionally, a second primer upstream promoter sequence that is non-complementary to a nucleic acid containing said target nucleic acid sequence or its complement, said 3′
terminal target-complementary sequence of said second primer being selected from the group consisting of SEQ ID NO;
4, SEQ ID NO;
5, SEQ ID NO;
6, and SEQ ID NO;
7, allowing for the presence of RNA equivalents and nucleotide analogs; anda molecular beacon probe, the base sequence of which consists of a target-complementary sequence and a pair of mutually complementary base sequences that are non-complementary to a nucleic acid containing said target nucleic acid sequence or its complement, said target-complementary sequence of said probe being selected from the group consisting of SEQ ID NO;
23, SEQ ID NO;
24 and SEQ ID NO;
25, allowing for the presence of DNA equivalents and nucleotide analogs. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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Specification