Light irradiation method, light irradiation device, and fine particle analyzing apparatus
First Claim
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1. A light irradiation method of irradiating a specimen moving through a flow channel with directional light, the method comprising:
- irradiating the specimen with the directional light from a first light source while performing scanning using the directional light in a widthwise direction of the flow channel, the directional light having an at least substantially circular irradiation spot having a diameter that is smaller than a width of the flow channel,obtaining positional information of the specimen prior to irradiating the specimen with the directional light by performing scanning of the specimen with a second irradiation spot from a second light source, and detecting a measurement light that is generated by the irradiation of the specimen by the second light source,wherein irradiating the specimen with the directional light at a given time is based on the obtained positional information of the specimen, andwherein scanning is performed in the widthwise direction of the flow channel at a single position in a flow channel direction a plurality of times during a period of time when the specimen moves through an area irradiated by the irradiation spot.
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Abstract
A light irradiation method of irradiating a specimen in a flow channel with directional light includes the step of irradiating the specimen with the directional light while performing scanning using the directional light in a widthwise direction of the flow channel. The directional light has an irradiation spot that is smaller than a width of the flow channel. Accordingly, energy density of the irradiation spot can be increased without increasing output power of a light source.
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14 Claims
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1. A light irradiation method of irradiating a specimen moving through a flow channel with directional light, the method comprising:
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irradiating the specimen with the directional light from a first light source while performing scanning using the directional light in a widthwise direction of the flow channel, the directional light having an at least substantially circular irradiation spot having a diameter that is smaller than a width of the flow channel, obtaining positional information of the specimen prior to irradiating the specimen with the directional light by performing scanning of the specimen with a second irradiation spot from a second light source, and detecting a measurement light that is generated by the irradiation of the specimen by the second light source, wherein irradiating the specimen with the directional light at a given time is based on the obtained positional information of the specimen, and wherein scanning is performed in the widthwise direction of the flow channel at a single position in a flow channel direction a plurality of times during a period of time when the specimen moves through an area irradiated by the irradiation spot. - View Dependent Claims (2, 3, 7, 8, 9, 10, 11, 12, 13, 14)
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4. A light irradiation device that irradiates a specimen moving through a flow channel with directional light, the device comprising at least:
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a first light source used to perform the irradiation with light having an at least substantially circular irradiation spot having a diameter that is smaller than a width of the flow channel; a first scanning means for performing scanning using the light from the first light source in a widthwise direction of the flow channel, a second light source; a second scanning means for performing scanning of the specimen with a second irradiation spot from the second light source, and a detector used to detect a measurement light that is generated by the irradiation of the specimen by the second light source, wherein the measurement light is used to obtain positional information of the specimen prior to irradiating the specimen with the directional light; wherein irradiating the specimen with the directional light at a given time is based on the obtained positional information of the specimen, and wherein the first scanning means performs scanning in the widthwise direction of the flow channel at a single position in a flow channel direction a plurality of times during a period of time when the specimen moves through an area irradiated by the irradiation spot. - View Dependent Claims (5)
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6. A light irradiation device that irradiates a specimen moving through a flow channel with directional light, the device comprising at least:
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a first light source used to perform the irradiation with light having an at least substantially circular irradiation spot having a diameter that is smaller than a width of the flow channel; a first scanner that performs scanning using the light from the first light source in a widthwise direction of the flow channel, a second light source; a second scanner for performing scanning of the specimen with a second irradiation spot from the second light source, and a detector used to detect a measurement light that is generated by the irradiation of the specimen by the second light source, wherein the measurement light is used to obtain positional information of the specimen prior to irradiating the specimen with the directional light; wherein irradiating the specimen with the directional light at a given time is based on the obtained positional information of the specimen, and wherein the first scanner performs scanning in the widthwise direction of the flow channel at a single position in a flow channel direction a plurality of times during a period of time when the specimen moves through an area irradiated by the irradiation spot.
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Specification