Device for the determination of glycated hemoglobin
First Claim
1. A device for use in electrochemically assaying glycated hemoglobin in a blood, blood-containing, or blood-derived sample, comprising:
- a first chamber and a second chamber separate from the first chamber, wherein the first and second chambers comprise;
a cathode comprising an enzyme capable of reducing oxygen to water and a redox mediator, wherein the enzyme and the redox mediator are immobilized on the cathode by a polymer, wherein the redox mediator and the polymer form a redox polymer, and an anode, andwherein the second chamber further comprises a surface-immobilized glycated hemoglobin separating agent or a glycated hemoglobin hydrolysis agent.
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Accused Products
Abstract
A method of determining the percentage of glycated hemoglobin in a blood sample is disclosed wherein at least one of the assay steps is performed electrochemically. The method includes determining the total amount of hemoglobin in a sample by electrochemically measuring, in an oxygen electroreduction reaction at a cathode, the amount of oxygen in the sample. Because the amount of oxygen dissolved in the sample is known, the total hemoglobin is determined by subtracting the amount of free oxygen from the total oxygen measured, recognizing the fast equilibrium Hb+O2⇄HbO2. This can be followed by determining the amount of glycated hemoglobin in the sample. The cathode reaction is accomplished by contacting the sample with an enzyme, the enzyme being a copper-containing enzyme having four copper ions per active unit. The family of these enzymes includes, for example, laccases and bilirubin oxidases. A device associated with such a process or method is also provided.
60 Citations
21 Claims
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1. A device for use in electrochemically assaying glycated hemoglobin in a blood, blood-containing, or blood-derived sample, comprising:
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a first chamber and a second chamber separate from the first chamber, wherein the first and second chambers comprise; a cathode comprising an enzyme capable of reducing oxygen to water and a redox mediator, wherein the enzyme and the redox mediator are immobilized on the cathode by a polymer, wherein the redox mediator and the polymer form a redox polymer, and an anode, and wherein the second chamber further comprises a surface-immobilized glycated hemoglobin separating agent or a glycated hemoglobin hydrolysis agent. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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Specification