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Evolving new molecular function

  • US 8,206,914 B2
  • Filed: 07/12/2010
  • Issued: 06/26/2012
  • Est. Priority Date: 08/19/2002
  • Status: Active Grant
First Claim
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1. An in vitro method of enriching a product of a nucleic acid-templated synthesis, the method comprising the steps of:

  • (a) providing a first library of molecules comprising a plurality of reaction products of a nucleic acid templated synthesis, which are not nucleic acids, wherein each reaction product is covalently attached to a corresponding oligonucleotide that templated the synthesis of the reaction product, and wherein each oligonucleotide comprises a nucleotide sequence indicative of the reaction product associated therewith, and wherein a portion of said reaction products are capable of binding to a preselected binding moiety;

    (b) exposing said first library of molecules to said binding moiety under conditions to permit reaction product capable of binding said binding moiety to bind thereto, wherein the reaction product has a Kd for the binding moiety of no less than 0.9 nM;

    (c) removing unbound reaction products; and

    (d) eluting bound reaction product from said binding moiety to produce a second library of molecules enriched at least 50-fold for reaction product that binds said binding moiety relative to said first library.

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