Photo-damage method for sorting particles
DC CAFCFirst Claim
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1. A method of sorting a mixture of stained sperm cells having either characteristic A or characteristic B into at least one population, the method comprising the steps of:
- a. flowing a fluid stream containing stained sperm cells through a flow path at a fluid delivery rate;
b. exciting fluorescence emissions from the stained sperm cells having characteristic A and the stained sperm cells having characteristic B flowing in the flow path;
c. detecting the fluorescence emissions from the excited sperm cells;
d. classifying the stained sperm cells as either having characteristic A or having characteristic B based upon the fluorescence emissions;
e. selecting stained sperm cells in the flow path based on their classification; and
f. photo-damaging the selected sperm cells to produce an enriched population of sperm with respect to either characteristic A or characteristic B.
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Abstract
A system and method for sorting a mixture of stained particles in a fluid flow path, including stained particles from two distinguishable groups. The system and method can include an electromagnetic radiation source for exciting fluorescence emissions from the stained particles, a photodetector for detecting the fluorescence emissions from the stained particles, a processor for classifying the stained particles; and a photo-damaging laser for damaging selected particles in the flow path.
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Citations
10 Claims
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1. A method of sorting a mixture of stained sperm cells having either characteristic A or characteristic B into at least one population, the method comprising the steps of:
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a. flowing a fluid stream containing stained sperm cells through a flow path at a fluid delivery rate; b. exciting fluorescence emissions from the stained sperm cells having characteristic A and the stained sperm cells having characteristic B flowing in the flow path; c. detecting the fluorescence emissions from the excited sperm cells; d. classifying the stained sperm cells as either having characteristic A or having characteristic B based upon the fluorescence emissions; e. selecting stained sperm cells in the flow path based on their classification; and f. photo-damaging the selected sperm cells to produce an enriched population of sperm with respect to either characteristic A or characteristic B. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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Specification