Methods of attaching biological compounds to solid supports using triazine

• US 8,207,332 B2
• Filed: 07/11/2011
• Issued: 06/26/2012
• Est. Priority Date: 12/17/2003
• Status: Active Grant

• Current Assignee: Illumina Incorporated
• Original Assignee: Illumina Incorporated
• Inventors: Zhao, Chanfeng, Kozlov, Igor, Steinberg-Tatman, Galina
• Primary Examiner(s): Riley, Jezia
• Application Number:
  US13/180,439
• Publication Number:
  US 20110268903A1
• Time in Patent Office: 351 Days
• Field of Search: 544/1, 544/2, 435/6.1
• US Class Current: 544/1
• CPC Class Codes: C07D 251/12 having three double bonds between ring members or between ring members and non-ring members
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C07H 21/04 with deoxyribosyl as saccharide radical
• CPC Class Codes: Y10T 428/139 Open-ended, self-supporting conduit, cylinder, or tube-type article

Abstract

Disclosed are methods of attaching biologically active compounds to a solid surface, comprising modifying the solid surface using triazine chloride and attaching the biologically active compound to the triazine moiety.

Claims

1. A compound of Formula XX

2. The compound of claim 1, wherein Q is selected from the group consisting of resin, microbead, glass, controlled pore glass (CPG), polymer support, membrane, paper, plastic, plastic tube or tablet, plastic bead, glass bead, slide, ceramic, silicon chip, multi-well plate, nylon membrane, and a PVDF membrane.

3. The compound of claim 1, wherein Q comprises a PVDF membrane.

4. The compound of claim 1, wherein Q comprises plastic.

5. The compound of claim 1, wherein Q comprises glass.

6. The compound of claim 1, wherein Q comprises a silicon chip.

7. The compound of claim 1 wherein V comprises NH.

8. The compound of claim 1, wherein V comprises a combination of NH and (CH₂)_p.

9. The compound of claim 8, wherein V further comprises SiO₃.

10. The compound of claim 1, wherein X¹ is derivatized with a linker and DNA.

11. The compound of claim 1, wherein X¹ is derivatized with a linker and one or more non-naturally occurring nucleotides.

12. The compound of claim 1, wherein X¹ is derivatized with a linker and a polypeptide.

13. The compound of claim 1, wherein X¹ is derivatized with a linker and one or more non-naturally occurring amino acids.