Extraction of polar lipids by a two solvent method

• US 8,211,308 B2
• Filed: 11/01/2011
• Issued: 07/03/2012
• Est. Priority Date: 04/06/2010
• Status: Expired due to Fees

• Current Assignee: Heliae Development, LLC (PT Perusahaan Perkebunan London Sumatra Indonesia Tbk)
• Original Assignee: Heliae Development, LLC (PT Perusahaan Perkebunan London Sumatra Indonesia Tbk)
• Inventors: Kale, Aniket
• Primary Examiner(s): Drodge, Joseph
• Application Number:
  US13/286,889
• Publication Number:
  US 20120053357A1
• Time in Patent Office: 245 Days
• Field of Search: 210/633, 210/634, 210/639, 210/743, 210/749, 210/774, 210/806, 210/651, 210/770, 210/787, 210/804, 435/134, 435/262, 435/267, 435/271, 435/272, 443/07, 446/05, 47/14, 554/12, 554/14, 554/20, 208/15, 208/49, 208/87, 208/133, 208/142, 203/39
• US Class Current: 210/634
• CPC Class Codes: C11B 1/10 by extracting
• CPC Class Codes: C11B 1/108 after-treatment, e.g. of miscellae
• CPC Class Codes: C12P 7/6463 obtained from glyceride producing microorganisms, e.g. single cell oil
• CPC Class Codes: C12P 7/649 Biodiesel, i.e. fatty acid alkyl esters
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

A method for separating polar lipids from plant material, in particular, intact algal cells, using an amphipathic solvent set and a hydrophobic solvent set. Some embodiments include dewatering intact algal cells and then extracting polar lipids from the algal cells. The methods provide for single and multistep extraction processes which allow for efficient separation of algal polar lipids from a wet algal biomass while avoiding emulsification of extraction mixtures. These polar lipids are high value products which can be used as surfactants, detergents, and food additives. Neutral lipids remaining in the algal biomass after extraction of polar lipids can be used to generate renewable fuels.

Claims

1. A method of separating polar lipids from intact algal cells, comprising:

providing a wet algal culture comprising intact algal cells which comprise polar lipids;

dewatering the wet algal culture by removing extracellular water to increase the solid content of the wet algal culture to between about 5% w/w and about 50% w/w to result in a partially dewatered wet algal biomass;

mixing the partially dewatered wet algal biomass with an amphipathic solvent set, wherein the weight of the amphipathic solvent set is about the same as the weight of the partially dewatered wet algal biomass, and a hydrophobic solvent set to generate an extraction mixture comprising a heavier phase and a lighter phase, wherein the heavier phase comprises the amphipathic solvent set and partially dewatered wet algal biomass that comprises intact algal cells and the lighter phase comprises the hydrophobic solvent set and polar lipids;

separating the heavier phase of the extraction mixture from the lighter phase of the extraction mixture; and

separating the polar lipids from the lighter phase to generate a polar lipids fraction.

2. The method of claim 1 wherein dewatering is carried out by centrifuging, filtering, settling or float fractionating.

3. The method of claim 1 wherein the amphipathic solvent set is added in an amount to adjust the polarity index of the extraction mixture to between about 6.5 to 6.7 prior to the addition of the hydrophobic solvent set.

4. The method of claim 3 wherein the hydrophobic solvent set is added in an amount to adjust the polarity index of the extraction mixture to between about 5.7 to 5.9 after the addition of the amphipathic solvent set.

5. The method of claim 1 wherein the amphipathic solvent set is acetone, methanol, ethanol, isopropanol, butanone, dimethyl ether, and propionaldehyde. 2-propanol, acetonitrile, t-butyl alcohol, 1-propanol, water, heavy (D2O), ethylene glycol, glycerin, or a combination thereof.

6. The method of claim 1 wherein the hydrophobic solvent set is propane, butane, pentane, butene, propene, naphtha, an alkane, hexane, pentane, heptane, octane, an ester, ethyl acetate, butyl acetate), a ketone, methyl ethyl ketone, methyl isobutyl ketone, an aromatic, toluene, benzene, cyclohexane, tetrahydrofuran, a haloalkane, chloroform, trichloroethylene, an ether, diethyl ether, diesel, jet fuel, gasoline, or a combination thereof.

7. The method of claim 1, wherein separating the heavier phase from the lighter phase includes decanting, membrane filtering or centrifuging.

8. The method of claim 1 wherein separating the polar lipids from the lighter phase includes evaporation or distillation.

9. The method of claim 1 further comprising recovering the hydrophobic solvent set.

10. The method of claim 1 further comprising condensing and recovering the hydrophobic solvent set.

11. The method of claim 1, wherein the extraction mixture is heated.

12. The method of claim 11, wherein the extraction mixture is heated with microwaves, water, steam, hot oil or electricity.

13. The method of claim 1, wherein the extraction mixture is heated in a pressurized reactor.

14. The method of claim 13, wherein the pressurized reactor is a batch or a continuous reactor.

15. The method of claim 14, wherein the extraction mixture is heated with microwaves, water, steam, hot oil or electricity.

16. The method of claim 1, wherein the extraction mixture is heated at atmospheric pressure.

17. The method of claim 16, wherein the extraction mixture is heated with microwaves, water, steam, hot oil or electricity.