Diagnostic detection device
First Claim
1. A device for detecting an analyte in a liquid sample deposited on a first portion of the device for transport to a second portion of the device that is in fluid contact with the first portion, wherein the device comprises:
- A) in the first portion thereof, a labeled conjugate comprising a binding member reactive with a first epitope of the analyte and a label comprising colloidal gold particles having a mean particle size of at least about 50 nm prior to formation of the labeled conjugate; and
B) in the second portion thereof, a capture component that is directly or indirectly reactive with a second epitope of the analyte.
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Abstract
The invention comprises a device for detecting an analyte in a liquid sample deposited on a first portion of the device for transport to a second portion of the device that is in fluid contact with the first portion. In specific embodiments, the device comprises a labeled conjugate comprising a binding member reactive with a first epitope of the analyte and a label comprising a gold colloid, preferably having a mean particle size of 50 nm to 100 nm. In further embodiments, the device comprises a capture component comprising polymerized streptavidin. The diagnostic device is particularly useful in the preparation of pregnancy test kits.
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Citations
18 Claims
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1. A device for detecting an analyte in a liquid sample deposited on a first portion of the device for transport to a second portion of the device that is in fluid contact with the first portion, wherein the device comprises:
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A) in the first portion thereof, a labeled conjugate comprising a binding member reactive with a first epitope of the analyte and a label comprising colloidal gold particles having a mean particle size of at least about 50 nm prior to formation of the labeled conjugate; and B) in the second portion thereof, a capture component that is directly or indirectly reactive with a second epitope of the analyte. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A method for determining the presence of an analyte in a liquid sample comprising:
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A) providing an analyte detection device comprising a substrate with a first portion comprising colloidal gold having a mean particle size of at least about 50 nm releasably immobilized thereon and a second portion in fluid communication with the first portion and comprising a capture component immobilized thereon; B) adding a liquid sample to the first portion of the device; C) allowing the liquid sample to flow across the first portion and the second portion of the substrate; and D) determining the presence of the analyte in the liquid sample by visual inspection of the second portion of the substrate, wherein the presence of the analyte is indicated by the presence of color development in the area wherein the capture component is immobilized caused by accumulation of the colloidal gold. - View Dependent Claims (9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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Specification