Modified nucleosides and nucleotides and uses thereof
First Claim
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1. A modified nucleoside, comprising a base attached to a fluorophore through a cleavable linking group, wherein the cleavable linking group comprises a cleavable functionality and a hydrophilic spacer group of 5 to 150 atoms.
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Abstract
The invention is directed to modified guanine-containing nucleosides and nucleotides and uses thereof. More specifically, the invention relates to modified fluorescently labelled guanine-containing nucleosides and nucleotides which exhibit enhanced fluorophore intensity by virtue of reduced quenching effects.
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19 Claims
- 1. A modified nucleoside, comprising a base attached to a fluorophore through a cleavable linking group, wherein the cleavable linking group comprises a cleavable functionality and a hydrophilic spacer group of 5 to 150 atoms.
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19. A reagent for modifying the base of a nucleoside, comprising a fluorophore and a cleavable linking group, wherein the linking group comprises a cleavable functionality and a hydrophilic spacer group of 5 to 150 atoms.
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