Corn plant Mon88017 and compositions and methods for detection thereof

• US 8,212,113 B2
• Filed: 12/14/2004
• Issued: 07/03/2012
• Est. Priority Date: 12/15/2003
• Status: Active Grant

• Current Assignee: Monsanto Technology LLC (Bayer AG)
• Original Assignee: Monsanto Technology LLC (Bayer AG)
• Inventors: Beazley, Kim A., Coombe, Timothy R., Groth, Mark E., Hinchey, Terri B., Pershing, Jay C., Vaughn, Ty T., Zhang, Bei
• Primary Examiner(s): Kruse, David H
• Application Number:
  US10/582,007
• Publication Number:
  US 20080028482A1
• Time in Patent Office: 2,758 Days
• Field of Search: None
• US Class Current: 800/300.1
• CPC Class Codes: C12N 15/8275 Glyphosate
• CPC Class Codes: C12N 15/8286 for insect resistance
• CPC Class Codes: C12Q 1/6895 for plants, fungi or algae
• CPC Class Codes: C12Q 2600/13 Plant traits
• CPC Class Codes: Y02A 40/146 Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

The present invention provides a corn plant designated MON880 7 and DNA compositions contained therein. Also provided are assays for detecting the presence of the corn plant MON88017 based on a DNA sequence and the use of this DNA sequence as a molecular marker in a DNA detection method.

Claims

1. A glyphosate tolerant corn plant comprising DNA encoding a CRY3Bb1 protein and a CP4 EPSPS protein, said comprising SEQ ID NO:

1 and SEQ ID NO;

2.

6. The corn plant of claim 1 comprising SEQ ID NO:

3 and SEQ ID NO;

4.

17. The corn plant of claim 1 comprising SEQ ID NO:

3.

18. The corn plant of claim 1 comprising SEQ ID NO:

4.

19. A seed that produces the corn plant of claim 1.

2. A hybrid corn seed wherein at least one parent comprises corn event MON88017.

3. A seed of a corn plant comprising event MON88017, a representative sample of seed comprising said event having been deposited under ATCC Accession No. PTA-5582.

4. A corn plant comprising event MON88017 or parts thereof produced by growing the seed of claim 3.

5. Pollen, ovule, seed, roots, or leaves of the corn plant of claim 4.

7. A biological sample selected from the group consisting of corn oil, corn meal, corn flour, corn gluten, corn cakes, and corn starch, comprising a sufficient level of a nucleotide selected from the group consisting of SEQ ID NO:

1 and SEQ ID NO;

2, wherein the detection of said nucleotide in said sample is diagnostic for the presence of corn event MON88017 in said sample.

8. A corn plant, seed, or parts thereof, comprising corn event MON88017.

9. A composition derived from the corn plant, or parts thereof, of claim 8, said composition comprising corn event MON88017, wherein said composition is a commodity product selected from the group consisting of corn meal, corn flour, corn oil, corn silk, corn starch, and processed foodstuffs.

10. A method of producing an insect and glyphosate resistant corn plant, comprising:

(a) sexually crossing a first parent corn plant according to claim 8 and a second parent corn plant that lacks insect and glyphosate resistance, thereby producing a plurality of progeny plants; and

(b) selecting a progeny plant that is insect and glyphosate resistant by analyzing for the presence of at least one nucleotide sequence of SEQ ID NO;

1 and SEQ ID NO;

2.

11. The method of claim 10, wherein said selecting step (b) comprises (i) subjecting the progeny plant to a nucleic acid amplification reaction, wherein progeny plant that produces an amplicon comprising at least one nucleotide sequence of SEQ ID NO:

1 and SEQ ID NO;

2 is selected;

or (ii) subjecting the progeny plant to a nucleic acid hybridization reaction, wherein progeny plant hybridizing to a probe that hybridizes under stringent conditions with one or more DNA sequence selected from SEQ ID NO;

1 and SEQ ID NO;

2 is selected.

12. The method of claim 10, further comprising backcrossing the progeny plant that is insect and glyphosate resistant to the second parent corn plant, thereby producing a plant that is insect and glyphosate resistant.

13. A method for protecting a corn plant from insect infestation, comprising providing in the diet of a Coleopteran pest of corn an insecticidally effective amount of cell(s) or tissue(s) of the corn plant, or parts thereof, of claim 8.

14. The method of claim 13, wherein said Coleopteran pest is corn rootworm.

15. A method for controlling weeds in a field of corn plants according to claim 8, comprising applying an effective amount of a glyphosate containing herbicide to said field of corn plants.

16. The method of claim 15, wherein

(i) said glyphosate containing herbicide is sprayed in said field, and

(ii) said amount does not damage said corn plants.