Microparticles measuring apparatus

• US 8,213,009 B2
• Filed: 05/14/2010
• Issued: 07/03/2012
• Est. Priority Date: 05/21/2009
• Status: Active Grant

• Current Assignee: Sony Corporation (Sony Group Corp.)
• Original Assignee: Sony Corporation (Sony Group Corp.)
• Inventors: Muraki, Yosuke, Kakuta, Masaya
• Primary Examiner(s): Ton, Tri T
• Application Number:
  US12/780,376
• Publication Number:
  US 20110069310A1
• Time in Patent Office: 781 Days
• Field of Search: 356335-339, 356/72, 356/39, 2504581-4612, 3405391-53929, 34087001-87017
• US Class Current: 356/335
• CPC Class Codes: G01N 15/14 Optical investigation techniques, e.g. flow cytometry

Abstract

Disclosed herein is a microparticles measuring apparatus including: an optical detecting section configured to direct a laser beam toward microparticles passing through a flow channel, detect light emanating from the microparticles, and convert the detected light into electrical signals; and a controlling unit configured to calculate the speed of the microparticles passing through the flow channel according to the electrical signals and suspend the supply of solution containing the microparticles when the calculated speed exceeds a prescribed limit.

Claims

1. A microparticles measuring apparatus comprising:

optical detecting means for;

(a) directing a laser beam toward microparticles passing through a flow channel,

(b) detecting light emanating from the microparticles, and

(c) converting the detected light into electrical signals; and

controlling means for;

(a) calculating a speed of the microparticles passing through the flow channel according to the electrical signals based on the speed being inversely proportional to pulse widths of said electrical signals,

(b) determining if the calculated speed exceeds a prescribed limit or is lower than the prescribed limit; and

(c) when the calculated speed exceeds the prescribed limit or is lower than the prescribed limit, suspending the supply of solution containing the microparticles.

2. The microparticles measuring apparatus as defined in claim 1, further comprising:

the flow channel being formed on a microchip;

supplying means for introducing into said flow channel a sample solution containing at least one of the microparticles and a sheath solution; and

discharging means for discharging said sample solution and said sheath solution from said flow channel.

3. The microparticles measuring apparatus as defined in claim 2, wherein said supplying means and said discharging means are equipped with a sensor to detect the flow rate of at least one of the sample solution and the sheath solution.

4. The microparticles measuring apparatus as defined in claim 3, further comprising image displaying means for displaying said sensor which has detected the flow rate exceeding or being lower than the prescribed limit.

5. The microparticles measuring apparatus as defined in claim 4, which suspends the action of said supplying means when the speed of microparticle passing through said flow channel exceeds or is lower than the prescribed limit and displays any one of said sensors which has detected the flow rate exceeding or being lower than the prescribed limit on said image displaying means.

6. A microparticles measuring apparatus comprising:

an optical detecting section configured to;

(a) direct a laser beam toward microparticles passing through a flow channel,

(b) detect light emanating from the microparticles, and

(c) convert the detected light into electrical signals; and

a controlling unit configured to;

(a) calculate the speed of the microparticles passing through the flow channel according to the electrical signals based on the speed being inversely proportional to pulse widths of said electrical signals,

(b) determine if the calculated speed exceeds a prescribed limit or is lower than the prescribed limit, and

(c) when the calculated speed exceeds the prescribed limit or is lower than the prescribed limit, suspend the supply of solution containing the microparticles.