Systems for rapid identification of pathogens in humans and animals
First Claim
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1. A system, comprising:
- a) a nucleic acid amplification component for amplifying a nucleic acid from an unknown bioagent to generate an amplicon comprising said nucleic acid flanked by primer sequences;
b) a base composition determination component comprising a computer processor configured to determine the number of, but not the nucleic acid gene sequence order of, A residues, C residues, T residues, G residues, U residues, analogs thereof and/or mass tag residues thereof of said amplicon; and
c) a bioagent identification component comprising a computer processor and a database comprising a plurality of base compositions of nucleic acid amplicons of known bioagents delineated by said primer sequences wherein said base compositions identify the number of, but not the nucleic acid gene sequence order of, A residues, C residues, T residues, G residues, U residues, analogs thereof and/or mass tag residues thereof and wherein said known bioagents comprise at least one bacterium and at least one virus, wherein said base composition identification component is configured to compare the base composition of the amplicon from the unknown bioagent with a said database of base compositions from known bioagents to identify the unknown bioagent.
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Abstract
“The present invention provides systems for: identifying pathogens in biological samples from humans and animals, resolving a plurality of etiologic agents present in samples obtained from humans and animals, determining detailed genetic information about such pathogens or etiologic agents, and rapid detection and identification of bioagents from environmental, clinical or other samples.”
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Citations
23 Claims
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1. A system, comprising:
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a) a nucleic acid amplification component for amplifying a nucleic acid from an unknown bioagent to generate an amplicon comprising said nucleic acid flanked by primer sequences; b) a base composition determination component comprising a computer processor configured to determine the number of, but not the nucleic acid gene sequence order of, A residues, C residues, T residues, G residues, U residues, analogs thereof and/or mass tag residues thereof of said amplicon; and c) a bioagent identification component comprising a computer processor and a database comprising a plurality of base compositions of nucleic acid amplicons of known bioagents delineated by said primer sequences wherein said base compositions identify the number of, but not the nucleic acid gene sequence order of, A residues, C residues, T residues, G residues, U residues, analogs thereof and/or mass tag residues thereof and wherein said known bioagents comprise at least one bacterium and at least one virus, wherein said base composition identification component is configured to compare the base composition of the amplicon from the unknown bioagent with a said database of base compositions from known bioagents to identify the unknown bioagent. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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Specification