Device and method for measuring analytes
First Claim
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1. An analyte sensing device, comprising:
- a housing; and
a composition located within the housing, said composition comprising;
a matrix consisting of a plurality of macroporous particles or beads;
at least one lectin derivatized to said macroporous particles or beads for thermodynamically stabilizing a functional binding activity, said at least one lectin having a binding specificity for glucose;
an analyte analog capable of being bound by the at least one lectin;
a first reporting moiety having a spectral response intensity substantially dependent upon the concentration of glucose within the housing; and
a first modulating moiety adapted for influencing the intensity of the spectral response;
wherein said particles or beads have lumens with an exclusion volume larger than the molecular weight of said at least one lectin and said analyte analog.
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Abstract
The present invention is directed to a system, device and method for measuring the concentration of an analyte in a fluid or matrix. A thermodynamically stabilized analyte binding ligand for use in the system, device and method is disclosed. The thermodynamically stabilized analyte binding ligand is resistant to degradation at physiological temperatures and its use within the device provides a minimally invasive sensor for monitoring the concentration of an analyte in a fluid or matrix as are present in the body of an animal.
34 Citations
51 Claims
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1. An analyte sensing device, comprising:
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a housing; and a composition located within the housing, said composition comprising; a matrix consisting of a plurality of macroporous particles or beads; at least one lectin derivatized to said macroporous particles or beads for thermodynamically stabilizing a functional binding activity, said at least one lectin having a binding specificity for glucose; an analyte analog capable of being bound by the at least one lectin; a first reporting moiety having a spectral response intensity substantially dependent upon the concentration of glucose within the housing; and a first modulating moiety adapted for influencing the intensity of the spectral response; wherein said particles or beads have lumens with an exclusion volume larger than the molecular weight of said at least one lectin and said analyte analog. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 51)
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19. An analyte sensing device, comprising:
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a housing; and a composition located within the housing, said composition comprising; at least one lectin having a binding activity, an analyte analog capable of being bound by the at least one lectin; a first reporting moiety having a spectral response intensity substantially dependent upon the concentration of glucose within the housing; a first modulating moiety adapted for influencing the intensity of the spectral response; and a matrix consisting essentially of a plurality of macroporous particles or beads having lumens, said particles or beads being in a fixed relationship to said at least one lectin for stabilizing said binding activity; wherein said plurality of macroporous particles or beads allow free diffusion of an analyte and of said analyte analog into and out of said lumens. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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33. An analyte sensing device comprising
a housing; - and
an analyte sensing composition located within the housing, said composition comprising; a matrix consisting essentially of a plurality of macroporous particles or beads substantially evenly distributed throughout said housing; at least one lectin derivatized to said macroporous particles or beads for thermodynamically stabilizing a functional binding activity; a first reporting moiety having a spectral response intensity substantially dependent upon the concentration of an analyte within the housing; and a first modulating moiety adapted for influencing the intensity of the spectral response. - View Dependent Claims (34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50)
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Specification