Tissue culture medium comprising transforming growth factor beta 1 and basic fibroblast growth factor
First Claim
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1. A culture medium which comprises transforming growth factor β
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1 (TGFβ
1) and basic fibroblast growth factor (bFGF), wherein said bFGF is provided at a concentration of at least 2 ng/ml, and wherein said TGFβ
1 is provided at a concentration range of 0.06-0.24 ng/ml. said culture medium being capable of maintaining pluripotent stem cell in an undifferentiated state.
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Abstract
The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells.
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13 Claims
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1. A culture medium which comprises transforming growth factor β
-
1 (TGFβ
1) and basic fibroblast growth factor (bFGF), wherein said bFGF is provided at a concentration of at least 2 ng/ml, and wherein said TGFβ
1 is provided at a concentration range of 0.06-0.24 ng/ml. said culture medium being capable of maintaining pluripotent stem cell in an undifferentiated state. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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1 (TGFβ
Specification