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Nucleic acid sequencing by selective excitation of microparticles

  • US 8,222,040 B2
  • Filed: 08/28/2007
  • Issued: 07/17/2012
  • Est. Priority Date: 08/28/2007
  • Status: Active Grant
First Claim
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1. A method for sequencing nucleic acid microparticles, the method comprising the steps of:

  • selectively exciting a calibration target by interference of a plurality of illumination beams;

    obtaining optical images of the excited calibration target;

    processing the optical images of the excited calibration target to generate a first optical image of the excited calibration target;

    predicting a second optical image of the excited calibration target based on first calibration parameters;

    generating second calibration parameters based on a difference between the first optical image and the second optical image;

    performing a sequencing reaction on the nucleic acid microparticles using one or more sequencing reagents, the nucleic acid particles being different from the calibration target;

    selectively exciting the nucleic acid microparticles in an excitation pattern using the second calibration parameters;

    obtaining optical images of the nucleic acid microparticles at a resolution insufficient to resolve individual microparticles; and

    processing the optical images of the nucleic acid microparticles using information on the excitation pattern to determine a presence or absence of at least one optical signature, the presence or absence of said at least one optical signature indicating sequence information of the nucleic acid microparticles.

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