Polypeptides having cellobiohydrolase activity and polynucleotides encoding same

• US 8,227,667 B2
• Filed: 02/27/2012
• Issued: 07/24/2012
• Est. Priority Date: 09/28/2007
• Status: Active Grant

• Current Assignee: Novozymes A/S (Novozymes)
• Original Assignee: Novozymes A/S (Novozymes)
• Inventors: Maiyuran, Suchindra, Harris, Paul, Ding, Hanshu, Brown, Kimberly
• Primary Examiner(s): NASHED, NASHAAT T
• Application Number:
  US13/405,988
• Publication Number:
  US 20120156755A1
• Time in Patent Office: 148 Days
• Field of Search: None
• US Class Current: 800/295
• CPC Class Codes: C11D 3/38645 containing cellulase
• CPC Class Codes: C12N 9/2437 Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
• CPC Class Codes: C12Y 302/01091 Cellulose 1,4-beta-cellobiosidase (3.2.1.91)

Abstract

The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Claims

1. An isolated polynucleotide comprising a nucleotide sequence that encodes a polypeptide having cellobiohydrolase activity, selected from the group consisting of:

(a) a polynucleotide encoding a polypeptide having cellobiohydrolase activity comprising an amino acid sequence having at least 99% identity to amino acids 18 to 842 of SEQ ID NO;

2;

(b) a polynucleotide encoding a polypeptide having cellobiohydrolase activity comprising amino acids 18 to 482 of SEQ ID NO;

2; and

(c) a polynucleotide encoding a polypeptide having cellobiohydrolase activity comprising nucleotides 52 to 1809 of SEQ ID NO;

1.

2. The isolated polynucleotide of claim 1, which is contained in plasmid pSMai182 which is contained in E. coli NRRL B-50059.

3. A nucleic acid construct comprising the polynucleotide of claim 1 operably linked to one or more control sequences that direct the production of the polypeptide in an expression host.

4. An expression vector comprising the nucleic acid construct of claim 3.

5. A recombinant host cell comprising the nucleic acid construct of claim 3.

6. A method of producing a polypeptide having cellobiohydrolase activity, comprising:

(a) cultivating the host cell of claim 5 under conditions conducive for production of the polypeptide; and

(b) recovering the polypeptide.

7. A method of producing a polypeptide having cellobiohydrolase activity, comprising:

(a) cultivating a transgenic plant or a plant cell comprising the polynucleotide of claim 1 under conditions conducive for production of the polypeptide; and

(b) recovering the polypeptide.

8. A transgenic plant, plant part or plant cell transformed with the polynucleotide of claim 1.