Irreversible electroporation and tissue regeneration

• US 8,231,603 B2
• Filed: 02/10/2010
• Issued: 07/31/2012
• Est. Priority Date: 02/10/2009
• Status: Active Grant

• Current Assignee: AngioDynamics, Inc.
• Original Assignee: AngioDynamics, Inc.
• Inventors: Hobbs, Eamonn P., Lovewell, James G., Pearson, Robert M.
• Primary Examiner(s): STIGELL, THEODORE J
• Application Number:
  US12/703,355
• Publication Number:
  US 20100204638A1
• Time in Patent Office: 902 Days
• Field of Search: 604 20- 21, 604/522, 604/501, 604506-507, 604/511, 606/41, 606/32
• US Class Current: 604/522
• CPC Class Codes: A61B 18/148 having a short, rigid shaft for accessing the inner body transcutaneously, e.g. for neurosurgery or arthroscopy
• CPC Class Codes: A61B 2018/00613 Irreversible electroporation
• CPC Class Codes: A61N 1/306 Arrangements where at least part of the apparatus is introduced into the body
• CPC Class Codes: A61N 1/327 for enhancing the absorption properties of tissue, e.g. by electroporation

Abstract

A method and device are herein described to treat a target region of tissue, using at least one energy delivery device coupled to a power source and positioned in a treatment position so as to irreversibly electroporate tissue to ablate a target region, and introduce regenerative materials into a treated region.

Claims

1. A method for treating a target region of tissue, comprising:

providing at least one energy delivery device, wherein the at least one energy delivery device is connectable to a power source, and wherein the at least one energy delivery device comprises;

a shaft capable of being positioned within the target region of tissue;

the shaft having a lumen adapted to receive at least one regenerative material for insertion into the target region of tissue; and

at least one electrode-positioned on the shaft and adapted to deliver electrical pulses to the target region of tissue in an amount sufficient to irreversibly electroporate the target region of tissue;

positioning the at least one energy delivery device in a treatment position;

applying power from the power source to said target region of tissue to irreversibly electroporate at least a portion of the target region of tissue;

ablating at least a portion of said target region of tissue; and

introducing the at least one regenerative material into the target region of tissue after the step of ablating.

2. The method of claim 1, wherein the step of positioning includes placing at least a portion of the energy delivery device on a surface of the target region of tissue.

3. The method of claim 1, wherein the step of positioning includes advancing at least a portion of the at least one energy delivery device into the target region of tissue.

4. The method of claim 1 wherein the step of introducing the at least one regenerative material includes releasing the at least one regenerative material through the at least one energy delivery device.

5. The method of claim 1, wherein the at least one energy delivery device involves at least one of a monopolar electrode, bipolar electrode, and an array of electrodes.

6. The method of claim 1, further comprising applying the method as at least part of a medical procedure that involves at least one of a percutaneous, a laparoscopic, and an open surgery.

7. The method of claim 1, wherein prior to the step of introducing the at least one regenerative material, the method further comprises releasing at least one factor to optimize the target region of tissue.

8. The method of claim 7, wherein the factor is at least one of water, ions, hormones, paracrine agents, pharmacological mediators, and vasoreactive elements.

9. The method of claim 1, wherein the at least one regenerative material is applied a sufficient number of times to effect at least one of regrowth, restructuring, and cellular repopulation in the target region of tissue.

10. The method of claim 1, wherein the target region of tissue is at least a portion of a tissue selected from the group comprising:

digestive, skeletal, muscular, nervous, endocrine, circulatory, reproductive, integumentary, lymphatic, urinary, and soft tissue.

11. The method of claim 1, wherein the target region of tissue is at least one of a vessel, a liver, a lung, a pancreas, and a prostate.

12. The method of claim 1, where the target region of tissue is at least one of benign, malignant, cancerous, neoplastic, preneoplastic, and tumorous tissue.

13. The method of claim 1, wherein the at least one regenerative material includes at least one of totipotent, pluripotent, multipotent, and unipotent cells.

14. The method of claim 1, wherein said at least one regenerative material is at least one of autogeneic, isogeneic, allogeneic, and xenogeneic.

15. The method of claim 1, wherein the at least one regenerative material includes at least a portion of a cell selected from the group comprising:

smooth muscle cells, epithelial cells, endothelial cells, adult stem cells, vascular endothelial cell precursor cells, and mesodermal stromal cells.

16. The method of claim 1, wherein the at least one regenerative material includes at least one cell that is the same cell type as the primary cell type of said target region of tissue.

17. The method of claim 1, wherein the at least one regenerative material includes at least a portion of a cell selected from the group comprising:

liver, lung, pancreas, and bone.

18. The method of claim 1, wherein the at least one regenerative material includes at least one of VEGF, cytokines, and anti-inflammatory agents, water, ions, hormones, paracrine agents, pharmacological mediators and vasoreactive elements.

19. The method of claim 1, wherein the at least one regenerative material is at least a portion of a molecule selected from the group comprising:

DNA, RNA, proteins, carbohydrates, sugars, lipids, enzymes, proteases, and steroid.

20. The method of claim 1, wherein the at least one regenerative material includes at least one of polysaccharides, proteoglycans, hyaluronic acid, collagen, fibronectin, elastin, laminin, and integrins.

21. A method for treating a target region of tissue, comprising:

providing at least one energy delivery device, wherein the at least one energy delivery device is connectable to a power source, wherein the at least one energy delivery device comprises;

a shaft capable of being positioned within the target region of tissue, the shaft having

a lumen adapted to receive at least one regenerative material for insertion into the target region of tissue; and

at least one electrode positioned on the shaft and adapted to deliver electrical pulses to the target region of tissue in an amount sufficient to irreversibly electroporate the target region of tissue;

positioning the at least one energy delivery device in a treatment position;

setting at least one parameter on the power source to provide power to nonthermally irreversibly electroporate the target region of tissue to achieve ablation;

applying power from the power source according to the parameter to the target region of tissue to irreversibly electroporate at least a portion of the target region of tissue;

introducing said at least one regenerative material into the target region of tissue after the step of applying power; and

withdrawing said at least one energy delivery device from the target region of tissue after the regenerative material has been introduced into the target region of tissue.