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Thermal cycling system

  • US 8,232,091 B2
  • Filed: 05/17/2007
  • Issued: 07/31/2012
  • Est. Priority Date: 05/17/2006
  • Status: Active Grant
First Claim
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1. A thermal cycler apparatus for performing a polymerase chain reaction (PCR) comprising:

  • a heat block having a top surface and a bottom surface, the heat block comprising;

    one or more receptacles located on the top surface and configured to receive one or more sample vessels;

    a container containing a liquid composition having a heat transfer coefficient of less than or equal to 5.0 W/m °

    K, and wherein the container comprises a closed system with respect to the liquid composition; and

    a stir bar in contact with the liquid composition and configured to actively mix the liquid composition;

    a heating element in thermal contact with the liquid composition; and

    a temperature control assembly in communication with the heating element.

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