Blood urea nitrogen (BUN) sensor
First Claim
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1. A method for assaying urea in a sample using a device, comprising:
- moving the sample through a conduit to a sensor in the device comprising immobilized urease and immobilized carbonic anhydrase, wherein the urease and the carbonic anhydrase are immobilized on at least a portion of a single electrode, wherein the sensor is selected from the group consisting of an ammonium ion sensor, a pH sensor, a carbon dioxide sensor, a bicarbonate sensor, and a conductivity sensor,contacting the sample suspected of containing urea with the sensor, anddetecting a chemical moiety with the sensor,wherein the device is configured to inhibit carbon dioxide exchange from the sample to an air space in a region of the sensor comprising immobilized urease and immobilized carbonic anhydrase.
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Abstract
A BUN (blood urea nitrogen) sensor containing immobilized carbonic anhydrase and immobilized urease for the in vitro detection of urea nitrogen in blood and biological samples with improved performance and precision characteristics.
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Citations
31 Claims
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1. A method for assaying urea in a sample using a device, comprising:
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moving the sample through a conduit to a sensor in the device comprising immobilized urease and immobilized carbonic anhydrase, wherein the urease and the carbonic anhydrase are immobilized on at least a portion of a single electrode, wherein the sensor is selected from the group consisting of an ammonium ion sensor, a pH sensor, a carbon dioxide sensor, a bicarbonate sensor, and a conductivity sensor, contacting the sample suspected of containing urea with the sensor, and detecting a chemical moiety with the sensor, wherein the device is configured to inhibit carbon dioxide exchange from the sample to an air space in a region of the sensor comprising immobilized urease and immobilized carbonic anhydrase. - View Dependent Claims (2, 3, 4, 5)
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6. A method for improving the sensitivity of a urea sensor, which sensor comprises a detector and immobilized urease, the method comprising:
- adding an effective amount of carbonic anhydrase to said sensor, wherein said urea sensor is in a conduit of a device configured to inhibit carbon dioxide exchange from a sample to an air space in a region of the sensor comprising immobilized urease and carbonic anhydrase.
- View Dependent Claims (7, 8, 9, 10, 11)
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12. A method for detecting urea in a sample, comprising:
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(a) moving a sample suspected of containing urea through a conduit of a device and into contact with a sensor comprising urease and carbonic anhydrase immobilized on at least a portion of said sensor in the device, wherein the urease and the carbonic anhydrase are immobilized on at least a portion of a single electrode; and (b) processing signals from the sensor with a detector system, wherein the device is configured to inhibit carbon dioxide exchange from the sample to an air space in a region of the sensor comprising immobilized urease and carbonic anhydrase. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31)
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Specification