Process for producing ethanol

• US 8,236,534 B2
• Filed: 05/05/2011
• Issued: 08/07/2012
• Est. Priority Date: 03/11/1999
• Status: Expired due to Fees

• Current Assignee: ZeaChem, Inc.
• Original Assignee: ZeaChem, Inc.
• Inventors: Verser, Dan, Eggeman, Timothy J.
• Primary Examiner(s): Lilling, Herbert J
• Application Number:
  US13/101,900
• Publication Number:
  US 20110275127A1
• Time in Patent Office: 460 Days
• Field of Search: 435/135, 435/140, 435/252.7, 435/139, 435/161, 435/162, 435/163, 562/600, 562/606, 562/608
• US Class Current: 435/135
• CPC Class Codes: C07C 29/149 with hydrogen or hydrogen-containing gases
• CPC Class Codes: C07C 31/08 Ethanol
• CPC Class Codes: C07C 67/08 by reacting carboxylic acids or symmetrical anhydrides with the hydroxy or O-metal group of organic compounds
• CPC Class Codes: C07C 69/14 of monohydroxylic compounds
• CPC Class Codes: C12P 7/06 Ethanol, i.e. non-beverage
• CPC Class Codes: C12P 7/14 Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
• CPC Class Codes: C12P 7/54 Acetic acid vinegar C12J
• CPC Class Codes: C12P 7/56 Lactic acid
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel
• CPC Class Codes: Y02P 20/10 Process efficiency

Abstract

A process for producing ethanol including a combination of biochemical and synthetic conversions results in high yield ethanol production with concurrent production of high value coproducts. An acetic acid intermediate is produced from carbohydrates, such as corn, using enzymatic milling and fermentation steps, followed by conversion of the acetic acid into ethanol using esterification and hydrogenation reactions. Coproducts can include corn oil, and high protein animal feed containing the biomass produced in the fermentation.

Claims

1. A method comprising:

a. culturing a microorganism to produce a dilute solution of a carboxylic acid salt, the cation of which is capable of producing an insoluble salt when combined with a weak acid;

b. acidifying the dilute carboxylic acid salt solution with a weak acid to produce a carboxylic acid and an insoluble salt of the cation and the weak acid;

c. esterifying the carboxylic acid with alcohol to form an ester; and

d. recovering the ester.

2. The method of claim 1, wherein the microorganism is cultured in fermentation medium comprising biomass.

3. The method of claim 2, wherein the biomass is selected from the group consisting of grass, trees, wood, paper, corn, rice, manure, crop residue, agricultural waste, and municipal waste.

4. The method of claim 3, wherein prior to fermentation, the biomass is treated using at least one treatment selected from the group consisting of heating and enzymatic hydrolysis.

5. The method of claim 1, wherein the microorganism is cultured using a fermentation medium that comprises carbohydrate.

6. The method of claim 5, wherein at least 60% of the carbon in the carbohydrate is converted into the ester.

7. The method of claim 1, wherein the carboxylic acid is selected from the group consisting of lactic acid and acetic acid.

8. The method of claim 1, wherein the cation is calcium.

9. The method of claim 1, wherein the weak acid is CO₂.

10. The method of claim 1, wherein the insoluble salt is a carbonate salt.

11. The method of claim 1, wherein the insoluble salt is calcium carbonate.

12. The method of claim 1, wherein the step of culturing comprises:

(i) producing lactic acid, lactate, or mixtures thereof, by fermentation; and

(ii) converting the lactic acid, lactate, or mixtures thereof, into acetic acid, acetate or mixtures thereof.

13. The method of claim 12, wherein the lactic acid is converted into acetic acid by fermentation.

14. The method of claim 1, wherein the step of culturing comprises using at least one homofermentative organism.

15. The method of claim 1, wherein the step of culturing comprises using at least one homoacetogenic organism.

16. The method of claim 1, wherein the step of culturing uses at least one organism from a genus selected from the group consisting of Lactobacillus, Clostridium, Acetobacterium, and Peptostreptococcus.

17. The method of claim 1, wherein the step of culturing uses at least one organism selected from the group consisting of Lactobacillus casei, Clostridium formicoaceticum, and Clostridium thermoaceticum.

18. The method of claim 1, wherein the alcohol is selected from the group consisting of methanol and ethanol.

19. The method of claim 1, further comprising hydrogenating the ester to produce alcohol.

20. The method of claim 19, wherein the hydrogen used for hydrogenation is produced by steam reforming.