Cell selection apparatus

• US 8,241,238 B2
• Filed: 05/15/2007
• Issued: 08/14/2012
• Est. Priority Date: 05/22/2006
• Status: Active Grant

• Current Assignee: Hamamatsu Photonics KK
• Original Assignee: Hamamatsu Photonics KK, Hamamatsu University School of Medichine
• Inventors: Hiruma, Teruo, Terakawa, Susumu, Iwai, Hidenao
• Primary Examiner(s): Deak, Leslie
• Assistant Examiner(s): MARCETICH, ADAM M
• Application Number:
  US12/301,483
• Publication Number:
  US 20090198168A1
• Time in Patent Office: 1,918 Days
• Field of Search: 604/5.02, 604/5.04, 604/6.01, 604/6.02, 604/6.03, 604/6.06, 604/6.08, 604/6.09, 604/6.16, 604/20, 600/309, 600/368, 600/481, 600/573, 209/3, 209/10, 209/12.1, 209/132, 209/422
• US Class Current: 604/6.08
• CPC Class Codes: A61M 1/36 Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
• CPC Class Codes: A61M 1/3678 Separation of cells using wave pressure; Manipulation of individual corpuscles
• CPC Class Codes: G01N 33/491 by separating the blood components G01N15/05 takes precedence

Abstract

A cell sorting apparatus includes an extracorporeal circulation system 10 for sampling blood from a subject and circulating the blood outside a body; a cell measuring section 20 for supplying measurement light to cells contained in the blood to optically measure cell information on the physical quantity relating to the volume of the cell itself or a nucleus of the cell; and a cell separating section 30 for separating cells with reference to the cell information measured. The cell separating section 30 separates cells, based on the cell information measured by the cell measuring section 20 and a sorting condition set for sorting a specific type of cell such as a cancer cell, the cells satisfying the sorting condition, and returns the other cells into the body via the extracorporeal circulation system 10. Thus, a cell sorting apparatus can be realized which is capable of suitably sorting a target cell from the cells in blood.

Claims

1. A cell sorting apparatus comprising:

an extracorporeal circulation system for sampling blood from a subject, circulating the blood outside a body and returning the blood into the body;

cell measuring means for supplying measurement light to cells contained in the blood sampled by the extracorporeal circulation system to optically measure cell information on the physical quantity relating to the volume of the cell itself or a nucleus of the cell, such that the cell measuring means irradiates a predetermined region of the blood containing the cell with the measurement light, acquires phase difference information by detecting light passing through the cell, and sets the phase difference information as the cell information;

cell separating means for separating, based on the cell information measured by the cell measuring means and a sorting condition set for sorting a specific type of cell, cells which satisfy the sorting condition to perform a first cell sorting, and returning the other cells into the body via the extracorporeal circulation system;

image acquiring means for acquiring an image of the cell separated by the cell separating means that satisfied the sorting condition; and

image analyzing means for extracting image data of a specific type of cell from the image acquired by the image acquiring means to perform a second cell sorting.

2. The cell sorting apparatus according to claim 1, further comprising separation instructing means for transmitting into the cell separating means a separation instructing signal indicating whether the cell satisfies the sorting condition, based on a result of comparison of the cell information measured by the cell measuring means and the sorting condition.

3. The cell sorting apparatus according to claim 1, further comprising displaying means for displaying an image of the cell acquired by the image acquiring means.

4. The cell sorting apparatus according to claim 1, wherein the image analyzing means further analyzes the image of the cell acquired by the image acquiring means with reference to previously prepared information of the specific type of cell, and deciding whether the cell is the specific type of cell.

5. The cell sorting apparatus according to claim 1, further comprising cell treating means for subjecting the cell, which is decided as the specific type of cell, to a predetermined treatment, and returning the treated cell into the body via the extracorporeal circulation system.

6. The cell sorting apparatus according to claim 5, wherein the cell treating means subjects the cell to homing treatment for making the cell express a homing function of directing the cell to a specific tissue.