Biosensor electrode mediators for regeneration of cofactors
First Claim
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1. A method of determining concentration of an analyte in a sample, the method comprising:
- applying the sample to a biosensor, wherein the biosensor comprises;
at least two substrates;
a plurality of electrodes comprising a fill indicator electrode, a working electrode, and a reference or counter/reference electrode; and
at least one reaction zone comprising an enzyme, a cofactor, and a mediator associated with at least the working electrode, wherein the mediator consists of one of the two following formulae;
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Abstract
The present invention is based on the discovery of NAD+ and NADP+ mediator compounds that do not bind irreversibly to thiol groups in the active sites of intracellular dehydrogenase enzymes. Such mediator compounds avoid a common mode of enzyme inhibition. The mediators can therefore increase the stability and reliability of the electrical response in amperometric electrodes constructed from NAD- or NADP-dependent enzymes.
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18 Claims
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1. A method of determining concentration of an analyte in a sample, the method comprising:
applying the sample to a biosensor, wherein the biosensor comprises; at least two substrates; a plurality of electrodes comprising a fill indicator electrode, a working electrode, and a reference or counter/reference electrode; and at least one reaction zone comprising an enzyme, a cofactor, and a mediator associated with at least the working electrode, wherein the mediator consists of one of the two following formulae; - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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