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Formation of immobilized biological layers for sensing

  • US 8,241,697 B2
  • Filed: 12/20/2007
  • Issued: 08/14/2012
  • Est. Priority Date: 12/20/2007
  • Status: Expired due to Fees
First Claim
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1. A method of forming a sensor comprising the steps of:

  • (a) forming a substantially homogeneous aqueous mixture comprising one or more enzymes, an acrylic-based monomer, a water soluble organic photo-initiator and a water soluble acrylic-based cross-linker in an aqueous mixture,(b) applying a controlled volume of said mixture onto a base sensor sufficient to cover said base sensor, and(c) exposing said applied volume to sufficient UV radiation to form an immobilized enzyme layer adhered to said base sensor;

    wherein the enzyme is urease and the base sensor is an ammonium ion-selective membrane.

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