Unsymmetrical cyanine dimer compounds and their application
First Claim
1. A complex comprising:
- a) a nucleic acid reporter molecule comprising a first nucleic acid complexing monomer moiety, a second nucleic acid complexing monomer moiety and a linker wherein the first nucleic acid complexing monomer moiety is covalently attached to the linker;
the second nucleic acid complexing monomer moiety is covalently attached to the linker; and
the linker is
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Abstract
Embodiments of the present invention provide methods and nucleic acid reporter molecules for the detection of nucleic acid in a sample. The nucleic acid reporter molecule comprises two unsymmetrical cyanine monomer moieties, which may be the same or different, that are covalently attached by a linker comprising at least one aromatic, heteroaromatic, cyclic or heterocyclic moiety comprising 3-20 non-hydrogen atoms selected from the group consisting of O, N, S, P and C. The linker may be rigid, relatively flexible or some degree thereof. The unsymmetrical cyanine monomer moieties comprise a substituted or unsubstituted benzazolium moiety and a substituted or unsubstituted pyridinium or quinolinium moiety that is connected by a methine bridge that is monomethine, trimethine or pentamethine. The linkers form the cyanine dimer compounds by attaching to the pyridinium or quinolinium moiety of the monomer moieties. The present nucleic acid reporter molecules find utility in forming a nucleic acid-reporter molecule complex and detecting the nucleic acid. In particular, present nucleic acid reporter molecules with a rigid linker and monomer moieties with a monomethine bridge find utility in detecting RNA in the presence of DNA.
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Citations
18 Claims
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1. A complex comprising:
a) a nucleic acid reporter molecule comprising a first nucleic acid complexing monomer moiety, a second nucleic acid complexing monomer moiety and a linker wherein the first nucleic acid complexing monomer moiety is covalently attached to the linker;
the second nucleic acid complexing monomer moiety is covalently attached to the linker; and
the linker is- View Dependent Claims (2, 3)
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4. A complex comprising:
a) a nucleic acid reporter molecule according to the formula - View Dependent Claims (5, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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6. A complex comprising:
a) a nucleic acid reporter molecule, wherein the molecule is
Specification