Integrated test element

• US 8,242,461 B2
• Filed: 08/29/2008
• Issued: 08/14/2012
• Est. Priority Date: 03/02/2006
• Status: Expired due to Fees

• Current Assignee: Roche Diabetes Care Inc. (Roche Holding AG)
• Original Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Inventors: Rösicke, Bernd, Hebestreit, Kai, Wehowski, Frederic, Gässler-Dietsche, Claudia
• Primary Examiner(s): Sakelaris, Sally
• Application Number:
  US12/231,119
• Publication Number:
  US 20090061076A1
• Time in Patent Office: 1,446 Days
• Field of Search: 250/458.1, 257/40
• US Class Current: 250/458.1
• CPC Class Codes: G01N 21/255 Details, e.g. use of specially adapted sources, lighting or optical systems
• CPC Class Codes: G01N 21/8483 Investigating reagent band test-element handling not specific to a test method G01N33/4875; analytical elements specific to chemical analysis of biological material G01N33/52; autometer with reagent band G01N35/04
• CPC Class Codes: G01N 2201/0628 Organic LED [OLED]

Abstract

An integrated test element (110) is proposed for detecting for detecting at least one analyte in a sample (142), in particular in a liquid sample (142). The integrated test element (110) has a carrier element (112), with an application face (114) on which at least one organic electroluminescent component (126) is applied. Furthermore, at least one indicator substance (136) is applied on the application face (114), which indicator substance (136) alters at least one optical characteristic, in particular an emission characteristic (e.g. a fluorescence characteristic), when it comes into contact with the at least one analyte. Furthermore, the integrated test element (110) has at least one photodetector element (116).

Claims

1. An integrated test element for detecting at least one analyte in a sample, in particular in a liquid sample, comprising:

a carrier element, wherein at least one organic electroluminescent component, in particular at least one organic electroluminescent thin-film component, in particular at least one organic light-emitting diode (OLED), is applied on an application face of the carrier element, wherein the integrated test element furthermore comprises at least one photodetector element, wherein the integrated test element comprises at least one indicator substance that is at least partially integrated into at least one organic layer of the at least one organic electroluminescent component, wherein the at least one organic layer is a layer of a sandwich structure of the organic electroluminescent component and is part of one or more organic layers which are embedded between electrodes of the organic electroluminescent component such that the indicator substance is positioned between the electrodes of the organic electroluminescent component, wherein the at least one organic electroluminescent component is designed in such a way that at least some of the excitation light emitted by it reaches the at least one indicator substance, wherein the at least one indicator substance is designed to alter at least one optical characteristic, in particular an emission characteristic, in particular a fluorescence characteristic, when the at least one indicator substance comes into contact with the at least one analyte, wherein the integrated test element furthermore comprises at least one reference device which is designed to alter at least one optical characteristic, in particular at least one of a reflection characteristic and an emission characteristic, in particular a fluorescence characteristic, depending on the intensity of the excitation light emitted by the at least one organic electroluminescent component, and wherein the alteration of the at least one optical characteristic is at least substantially independent of the presence or absence of the at least one analyte.

2. The integrated test element as claimed in claim 1, wherein the at least one photodetector element includes at least one organic thin-film photodetector element.

3. The integrated test element as claimed in claim 1, wherein the at least one photodetector element is arranged on the application face.

4. The integrated test element as claimed in claim 1, wherein the at least one indicator substance is merged into the at least one organic layer of the at least one organic electroluminescent component.

5. The integrated test element as claimed in claim 1, wherein the at least one carrier element includes one or more of a needle-shaped, cannula-shaped and lancet-shaped part.

6. The integrated test element as claimed in claim 1, wherein the excitation light emitted by the at least one organic electroluminescent component is at least partially prevented from reaching the at least one photodetector element.

7. The integrated test element as claimed in claim 6, further including one or more of an optical filter element and a light-blocker element.

8. The integrated test element as claimed in claim 1, wherein the at least one reference device includes at least one or both of a reflector and a reference fluorophore.

9. The integrated test element of claim 1, wherein at least one of the organic electroluminescent component and the photodetector element is composed of several individual organic electroluminescent components or photodetector elements, respectively, in particular of at least one matrix of such components.

10. The integrated test element of claim 1, wherein the at least one organic electroluminescent component and the at least one photodetector element are arranged in the same layer plane on the carrier element.

11. The integrated test element as claimed in claim 1, further comprising:

an additional barrier layer that at least partially covers the integrated test element, said barrier layer including at least one of the following properties;

an impermeability to the at least one indicator substance;

an at least partial permeability to the sample; and

an action separating the sample at least partially into its constituents.

12. The integrated test element as claimed in claim 1, wherein the at least one indicator substance is chemically bound with the at least one organic layer of the at least one organic electroluminescent component.

13. The integrated test element as claimed in claim 5, wherein the one or more of the needle-shaped, cannula-shaped and lancet-shaped part is configured for at least one of piercing of and insertion into a body tissue.

14. The integrated test element as claimed in claim 8, wherein the at least one reference device includes a paper brightener.

15. The integrated test element as claimed in claim 8, wherein the at least one reference device includes a metal oxide.

16. The integrated test element as claimed in claim 15, wherein the metal oxide includes TiO₂.

17. An integrated test element for detecting at least one analyte in a sample, comprising:

a carrier element including at least one organic light-emitting diode (OLED) applied on an application face of the carrier element, the at least one OLED including one or more organic layers positioned between opposite electrodes;

at least one photodetector element; and

at least one indicator substance positioned between the electrodes of the at least one OLED;

wherein the at least one OLED is configured to emit excitation light such that at least some of the excitation light reaches the at least one indicator substance, and the at least one indicator substance is designed to alter at least one optical characteristic when the at least one indicator substance comes into contact with the at least one analyte.

18. An integrated test element for detecting at least one analyte in a sample, comprising:

a carrier element including at least one organic light-emitting diode (OLED) applied on an application face of the carrier element, the at least one OLED including oppositely positioned electrodes and one or more organic layers;

at least one photodetector element; and

at least one indicator substance merged or chemically bound into at least one layer of the one or more organic layers of the at least one OLED, the at least one layer being positioned between the electrodes of the at least one OLED;

wherein the at least one OLED is configured to emit excitation light such that at least some of the excitation light reaches the at least one indicator substance, and at least one optical characteristic of the at least one indicator substance is alterable when the at least one indicator substance comes into contact with the at least one analyte.

19. The integrated test element of claim 18, wherein the at least one OLED is further configured such that the at least one indicator substance is surrounded by light-emitting molecules when the at least one OLED emits excitation light.

20. The integrated test element of claim 18, wherein the at least one OLED is further configured such that formation of the excitation light, reaction of the at least one indicator substance with the at least one analyte, and excitation of the at least one indicator substance by the excitation light occurs between the electrodes of the at least one OLED.

21. An integrated test element for detecting at least one analyte in a sample, comprising:

a carrier element including at least one organic light-emitting diode (OLED) applied on an application face of the carrier element, the at least one OLED including one or more organic layers positioned between opposite electrodes;

at least one photodetector element; and

at least one indicator substance positioned between the electrodes of the at least one OLED;

wherein the at least one OLED is configured to emit excitation light such that at least some of the excitation light reaches the at least one indicator substance and the at least one indicator substance is surrounded by light-emitting molecules when the at least one OLED emits excitation light, and at least one optical characteristic of the at least one indicator substance is alterable when the at least one indicator substance comes into contact with the at least one analyte.

22. The integrated test element of claim 21, wherein the at least one indicator substance is merged or chemically bound into at least one layer of the one or more organic layers of the at least one OLED, the at least one layer being positioned between the electrodes of the at least one OLED.

23. The integrated test element of claim 21, wherein the at least one OLED is further configured such that formation of the excitation light, reaction of the at least one indicator substance with the at least one analyte, and excitation of the at least one indicator substance by the excitation light occurs between the electrodes of the at least one OLED.

24. An integrated test element for detecting at least one analyte in a sample, comprising:

a carrier element including at least one organic light-emitting diode (OLED) applied on an application face of the carrier element, the at least one OLED including one or more organic layers positioned between opposite electrodes;

at least one photodetector element; and

at least one indicator substance;

wherein the at least one OLED is configured such that formation of excitation light, reaction of the at least one indicator substance with the at least one analyte, and excitation of the at least one indicator substance by the excitation light occurs between the electrodes of the at least one OLED, and at least one optical characteristic of the at least one indicator substance is alterable when the at least one indicator substance comes into contact with the at least one analyte.

25. The integrated test element of claim 24, wherein the at least one indicator substance is merged or chemically bound into at least one layer of the one or more organic layers of the at least one OLED, the at least one layer being positioned between the electrodes of the at least one OLED.

26. The integrated test element of claim 24, wherein the at least one OLED is further configured such that the at least one indicator substance is surrounded by light-emitting molecules upon formation of the excitation light.