Lighting design of high quality biomedical devices

• US 8,242,462 B2
• Filed: 01/21/2010
• Issued: 08/14/2012
• Est. Priority Date: 01/23/2009
• Status: Active Grant

• Current Assignee: Lumencor, Inc.
• Original Assignee: Lumencor, Inc.
• Inventors: Jaffe, Claudia B., Jaffe, Steven M., Conner, Arlie R.
• Primary Examiner(s): Porta, David
• Assistant Examiner(s): Maupin, Hugh H
• Application Number:
  US12/691,601
• Publication Number:
  US 20100187440A1
• Time in Patent Office: 936 Days
• Field of Search: 250/459.1, 250/458.1
• US Class Current: 250/459.1
• CPC Class Codes: G01J 1/58 using luminescence generated by light
• CPC Class Codes: G01J 3/10 Arrangements of light sources specially adapted for spectrometry or colorimetry
• CPC Class Codes: G01N 21/64 Fluorescence; Phosphorescence
• CPC Class Codes: G02B 6/0003 the light guides being doped with fluorescent agents

Abstract

The invention relates to a plurality of light sources to power a variety of applications including microarray readers, microplate scanners, microfluidic analyzers, sensors, sequencers, Q-PCR and a host of other bioanalytical tools that drive today'"'"'s commercial, academic and clinical biotech labs.

Claims

1. A solid-state light engine adapted to analyze a sample having one or more fluorescent tags comprising:

at least one light pipe;

at least one solid state light source;

one or more dichroic mirrors;

wherein the at least one solid state light source drives luminescence in the light pipe; and

wherein the at least one light pipe, the at least one solid state light source, and the one or more dichroic mirrors are used to produce a beam of excitation light having a plurality of colors, wherein the beam of excitation light is adapted to selectively detect the one or more fluorescent tags within the sample; and

wherein five solid state light sources are used with the one or more dichroic mirrors to create an eight-color beam of excitation light.

2. The solid-state light engine of claim 1, wherein the one or more solid state light sources are light-emitting diodes (LEDs).

3. The solid-state light engine of claim 1, wherein the eight-color beam of excitation light is a single coaxial beam.

4. The solid-state light engine of claim 1, wherein a plurality of light pipes and a plurality of solid state light sources have similar modulation capabilities, whereby the plurality of light pipes are tuned to different output wavelengths and adapted to selectively detect multiple fluorescent tags within the sample.

5. The solid-state light engine of claim 1, wherein each of the at least one solid state light sources are collimated so as to be efficiently combined.

6. The solid-state light engine of claim 1, further comprising one or more filter sliders to filter light from one or more of the at least one solid state light source.

7. The solid-state light engine of claim 1, wherein the at least one light pipe comprises one of a glass fiber doped with a rare earth metal, a glass fiber activated with a transition metal, and a polymer fiber doped with a dye.

8. The solid-state light engine of claim 1, wherein the light engine operates through a process of spontaneous emission.

9. The solid-state light engine of claim 1, wherein the light engine comprises relay optics having one or more optical fibers.

10. The solid-state light engine of claim 8, wherein the relay optics are constructed in a loop so that light can pass through one or more capillaries multiple times to enhance excitation efficiency.

11. The solid-state light engine of claim 1, comprising a plurality of light pipes, wherein the plurality of light pipes are coupled in parallel.

12. The solid-state light engine of claim 1, comprising a plurality of light pipes, wherein the plurality of light pipes are coupled in series.

13. A method for analyzing a sample comprising:

(a) pumping excitation light from one or more photo luminescent light pipes together with excitation light from one or more light emitting diodes to produce a beam of excitation light having one or more colors;

(b) directing the beam of excitation light onto the sample;

(c) directing fluorescence from the sample onto one or more fluorescence detectors; and

using five light emitting diodes with the one or more dichroic mirrors to create an eight-color beam of excitation light.

14. A system adapted to analyze a sample having one or more fluorescent tags comprising:

a light pipe engine, said light pipe engine comprising at least one light pipe, at least one solid state light source, and at least one or more dichroic mirrors, wherein the at least one solid state light source drives luminescence in the light pipe, wherein the at least one light pipe, the at least one solid state light source, and the one or more dichroic mirrors are used to produce a beam of excitation light having a plurality of colors, wherein the beam of excitation light is adapted to selectively detect the one or more fluorescent tags within the sample;

at least one filter attached to the light pipe engine that filters the beam of excitation light; and

an optical delivery pipe that is adapted to direct the beam of excitation light from the light pipe engine to the sample; and

wherein five solid state light sources are used with the one or more dichroic mirrors to create an eight-color beam of excitation light.