Compensator for multiple surface imaging
First Claim
1. A method for detecting features of an array, comprising:
- (a) providing a support structure comprising a first interior surface and a second interior surface that are in a generally mutually facing relation, wherein the first interior surface and the second interior surface are separated by a fluid passage, and wherein an array of features is distributed on the first interior surface and on the second interior surface, wherein the features are present at a density of at least 1,000 sites per square millimeter;
(b) detecting the array at a resolution to distinguish the features on the first interior surface; and
(c) detecting the array at a resolution to distinguish the features on the second interior surface.
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Accused Products
Abstract
A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.
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Citations
31 Claims
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1. A method for detecting features of an array, comprising:
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(a) providing a support structure comprising a first interior surface and a second interior surface that are in a generally mutually facing relation, wherein the first interior surface and the second interior surface are separated by a fluid passage, and wherein an array of features is distributed on the first interior surface and on the second interior surface, wherein the features are present at a density of at least 1,000 sites per square millimeter; (b) detecting the array at a resolution to distinguish the features on the first interior surface; and (c) detecting the array at a resolution to distinguish the features on the second interior surface. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. A method for detecting features of an array, comprising:
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(a) providing a support structure comprising a first interior surface and a second interior surface that are in a generally mutually facing relation, wherein the first interior surface and the second interior surface are separated by a fluid passage, and wherein an array of features is distributed on the first interior surface and on the second interior surface; (b) detecting the array at a resolution between 0.1 and 50 microns to distinguish the features on the first interior surface; and (c) detecting the array at a resolution between 0.1 and 50 microns to distinguish the features on the second interior surface. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31)
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Specification