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Thermal reaction device and method for using the same

  • US 8,247,178 B2
  • Filed: 10/14/2009
  • Issued: 08/21/2012
  • Est. Priority Date: 04/03/2003
  • Status: Active Grant
First Claim
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1. A microfluidic device for nucleic acid analysis, said device comprising a branched partitioning system comprisinga) a first flow channel in fluidic communication with a fluid inlet, said fluid inlet for introduction of a sample fluid comprising a nucleic acid;

  • b) a plurality of second flow channels branching from, and in fluidic communication with, said first flow channel; and

    c) a plurality of valves spaced along each second flow channel, positioned so that when said valves are closed said second flow channels are each partitioned into a plurality of equally sized adjacent fluidically isolated reaction sites, wherein each said reaction site of said plurality of reaction sites is separated from an adjacent reaction site by a single valve, wherein the isolated reaction chambers are not located at intersections of pairs of flow channels;

    wherein a sample fluid introduced into the inlet flows through the first flow channel into the second flow channels, and a sample fluid in a second flow channel can flow to the inlet only by passing through the first flow channel.

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