Thermal reaction device and method for using the same
First Claim
1. A microfluidic device for nucleic acid analysis, said device comprising a branched partitioning system comprisinga) a first flow channel in fluidic communication with a fluid inlet, said fluid inlet for introduction of a sample fluid comprising a nucleic acid;
- b) a plurality of second flow channels branching from, and in fluidic communication with, said first flow channel; and
c) a plurality of valves spaced along each second flow channel, positioned so that when said valves are closed said second flow channels are each partitioned into a plurality of equally sized adjacent fluidically isolated reaction sites, wherein each said reaction site of said plurality of reaction sites is separated from an adjacent reaction site by a single valve, wherein the isolated reaction chambers are not located at intersections of pairs of flow channels;
wherein a sample fluid introduced into the inlet flows through the first flow channel into the second flow channels, and a sample fluid in a second flow channel can flow to the inlet only by passing through the first flow channel.
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Abstract
An M×N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.
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Citations
11 Claims
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1. A microfluidic device for nucleic acid analysis, said device comprising a branched partitioning system comprising
a) a first flow channel in fluidic communication with a fluid inlet, said fluid inlet for introduction of a sample fluid comprising a nucleic acid; -
b) a plurality of second flow channels branching from, and in fluidic communication with, said first flow channel; and c) a plurality of valves spaced along each second flow channel, positioned so that when said valves are closed said second flow channels are each partitioned into a plurality of equally sized adjacent fluidically isolated reaction sites, wherein each said reaction site of said plurality of reaction sites is separated from an adjacent reaction site by a single valve, wherein the isolated reaction chambers are not located at intersections of pairs of flow channels; wherein a sample fluid introduced into the inlet flows through the first flow channel into the second flow channels, and a sample fluid in a second flow channel can flow to the inlet only by passing through the first flow channel. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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Specification