Device and method for multiple analyte detection
First Claim
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1. A method for reacting a target nucleic acid and detecting an optical signal, comprising:
- providing a substrate comprising a chamber, wherein the chamber is pre-loaded with a first unlabeled oligonucleotide such that the first unlabeled oligonucleotide is immobilized on the substrate, and the chamber is pre-loaded with a second unlabeled oligonucleotide such that the second unlabeled oligonucleotide is immobilized on the substrate;
introducing a target nucleic acid sequence into the chamber;
reacting the target nucleic acid sequence with both the first unlabeled oligonucleotide and the second unlabeled oligonucleotide in the chamber, to form a first reaction product in the chamber;
reacting the first reaction product with an optically detectable detection reagent in the chamber to form a second reaction product; and
detecting an optical signal produced in the chamber by the second reaction product in the chamber,wherein the substrate further comprises a channel in fluid communication with the chamber.
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Abstract
The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.
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16 Claims
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1. A method for reacting a target nucleic acid and detecting an optical signal, comprising:
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providing a substrate comprising a chamber, wherein the chamber is pre-loaded with a first unlabeled oligonucleotide such that the first unlabeled oligonucleotide is immobilized on the substrate, and the chamber is pre-loaded with a second unlabeled oligonucleotide such that the second unlabeled oligonucleotide is immobilized on the substrate; introducing a target nucleic acid sequence into the chamber; reacting the target nucleic acid sequence with both the first unlabeled oligonucleotide and the second unlabeled oligonucleotide in the chamber, to form a first reaction product in the chamber; reacting the first reaction product with an optically detectable detection reagent in the chamber to form a second reaction product; and detecting an optical signal produced in the chamber by the second reaction product in the chamber, wherein the substrate further comprises a channel in fluid communication with the chamber. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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Specification