Method and apparatus for biosensor spectral shift detection
First Claim
1. A method for determining biosensor resonance peak shifts, comprising the steps of:
- (i) obtaining a reference spectrum for the biosensor and a sample spectrum from the biosensor;
(ii) determining an initial offset estimate from the reference spectrum and the sample spectrum, the initial offset estimate being an estimate of the amount of resonance peak shift in the sample spectrum;
(iii) determining an incremental offset (shift) value; and
(iv) updating the initial offset estimate in response to the incremental offset (shift) value, the updated initial offset estimate representing an updated estimate of the amount of resonance peak shift.
1 Assignment
0 Petitions
Accused Products
Abstract
Performing high-resolution determination of the relative shift of the spectral properties of a biosensor. The shift in the resonance peak of the biosensor is indicative of the amount of material bound to the surface of the biosensor. A preferred biosensor is a Guided Mode Resonant Filter Biosensor (GMRFB). In one aspect of the invention, curve fitting is used to determine the relative location of the spectrum of the unexposed biosensor with respect to those spectra that are altered (e.g., shifted) by the presence of materials bound to the surface of the biosensor. In an alternative embodiment, the cross correlation function is used to detect spectral peak offsets between a reference spectrum and a spectrum measured from an exposed biosensor. In yet another alternative, maximal likelihood estimation techniques are used to determine the spectral shift or offs.
-
Citations
6 Claims
-
1. A method for determining biosensor resonance peak shifts, comprising the steps of:
-
(i) obtaining a reference spectrum for the biosensor and a sample spectrum from the biosensor; (ii) determining an initial offset estimate from the reference spectrum and the sample spectrum, the initial offset estimate being an estimate of the amount of resonance peak shift in the sample spectrum; (iii) determining an incremental offset (shift) value; and (iv) updating the initial offset estimate in response to the incremental offset (shift) value, the updated initial offset estimate representing an updated estimate of the amount of resonance peak shift. - View Dependent Claims (2, 3, 4, 5, 6)
-
Specification